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Vascular occlusion of the retina: an experimental model

I. Leukocyte aggregates

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Abstract

A new technique was developed to study the pathogenesis of retinal arteriolar occlusion in the pig. Using a catheter with its tip in front of the exit of the ophthalmic artery, autologous microparticles could be injected directly into the retinal arterial system. These microparticles were C5a-des-Arg stimulated leukocytes, which were aggregated to pellets of different sizes ranging from 0.26 to 1.0 mm. For better adhesion of the aggregates to the endothelium, endothelial damage was induced by the injection of additional substances, including methomidate and endothelial antibodies. In a further series of experiments systemic hypoxemic conditions were created over several hours prior to injection of the leukocyte aggregates. This resulted in cotton-wool spots and arterial branch occlusions with retinal edema. Furthermore, retinal hemorrhages occurred. This experimental model seems to be appropriate for mimicking retinal arteriolar occlusion syndromes.

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References

  1. Ashton N (1957) Retinal vascularization in health and disease. Am J Ophthalmol 44:7–17

    Google Scholar 

  2. Ashton N, Henkind P (1965) Experimental occlusion of retinal arterioles. Br J Opthalmol 49:225–234

    Google Scholar 

  3. Behrens-Baumann W, Scheurer G (1991) Morbus Purtscher. Variationsbreite der klinischen Manifestationen bei 11 Patienten und Überlegungen zur Pathogenese. Klin Monatsbl Augenheilkd 198:99–107

    Google Scholar 

  4. Behrens-Baumann W, Scheurer G, Schroer FH (1992) Pathogenesis of Purtscher's retinopathy. An experimental study. Graefe's Arch Clin Exp Ophthalmol 230

  5. Craddock PR (1979) The role of complement (C5a)-mediated granulocyte embolization in the pathogenesis of shock lung. Intensivmed Notfallmed Anaesth 16:81–87

    Google Scholar 

  6. Craddock PR, Fehr J, Brigham KL, Kronenberg RS, Jacob HS (1977) Complement and leukocyte-mediated pulmonary dysfunction in hemodialysis. N Engl J Med 296:769–774

    Google Scholar 

  7. Damerau B (1987) Biological activities of complement derived peptides. Rev Physiol Biochem Pharmacol 108:151–206

    Google Scholar 

  8. Damerau B, Grünefeld E, Vogt W (1980) Aggregation of leucocytes induced by the complement derived peptides C3a and C5a and by three synthetic formyl methionyl peptides. Int Arch Allergy Appl Immunol 63:159–169

    Google Scholar 

  9. Dollery CT, Henkind P, Paterson J, Ramalho PS, Hill DW (1965) Retinal microemboli: cine fluorescence angiography. Trans Ophthalmol Soc UK 85:271–273

    Google Scholar 

  10. Dollery CT, Henkind P, Paterson JW, Ramalho PS, Hill DW (1966) I. Ophthalmoscopic and circulatory changes in focal retinal ischemia. Br J Ophthalmol 50:285–324

    Google Scholar 

  11. Dollery CT, Henkind P, Paterson J, Ramalho PS (1967) Circulatory changes following retinal embolism by glass microscopheres. Bibl Anat 9:112–115

    Google Scholar 

  12. Gay AJ, Goldor H, Smith M (1964) Chorioretinal vascular occlusions with latex spheres. Invest Ophthalmol 3:674–656

    Google Scholar 

  13. Gee MH, Margiotta M, Tahamont von M, Flynn JT (1986) Free-radical generation in complement-induced pulmonary dysfunction. In: Taylor AE, Matalon S, Ward P (eds) Physiology of oxygen radicals. Bethesda, pp 187–197

  14. Goldor H, Gay AJ (1967) Chorioretinal vascular occlusions with latex microspheres (a long-term study), part II. Invest Ophthalmol 6:51–58

    Google Scholar 

  15. Henkind P (1966) Ballotini occlusion of retinal arteries. Collateral vessels. Br J Ophthalmol 50:482–495

    Google Scholar 

  16. Henkind P, Dollery CT (1966) Pathophysiology of the retinal vascular bed following acute embolization. Invest Ophthalmol 4:204–207

    Google Scholar 

  17. Herrenheiser J (1895)Über experimentelle Erzeugung von Embolien der Blutgefäße im Inneren des Auges. Klin Monatsbl Augenheilkd 33:315–322

    Google Scholar 

  18. Hirose K, Baba K (1963) A study of Takagashy-Onishi's (pulseless) disease; changes of the eye and brain of the rabbit produced by the injection of minute hair fragment suspension into the common carotid artery. Am J Ophthalmol 55:554–568

    Google Scholar 

  19. Hollenhorst RW (1962) Experimental embolization. Trans Am Ophthalmol Soc 60:233–237

    Google Scholar 

  20. Holtermann B (1975) Das Blutgefäßsystem des Kopfes beim Göttinger Miniaturschwein mit besonderer Berücksichtigung der venösen Anastomosen und Venenplexus. Inaugural-Dissertation, Ludwig-Maximilians-Univ., München

    Google Scholar 

  21. Jacob HS (1978) Granulocyte-complement interaction. Arch Int Med 138:461–463

    Google Scholar 

  22. Jacob HS, Goldstein IM, Shapiro I, Weissmann G (1978) Sudden blindness in acute pancreatitis: a manifestation of complement induced retinal leucostasis. Clin Res 26:498A

    Google Scholar 

  23. Jacob HS, Craddock PR, Hammerschmidt DE, Moldow CF (1980) Complement-induced granulocyte aggregation. N Engl J Med 302:789–794

    Google Scholar 

  24. Jacob HS, Goldstein IM, Shapiro I, Craddock PR, et al. (1981) Sudden blindness in acute pancreatitis. Arch Int Med 141:134–136

    Google Scholar 

  25. Klöti R (1967) Experimental occlusion of retinal and ciliary vessels in owl monkeys. Exp Eye Res 6:393–399

    Google Scholar 

  26. Kulvin SM, Noble JD (1967) Experimental retinal embolism. Arch Ophthalmol 78:774–787

    Google Scholar 

  27. Modes E (1936) Das Blutgefäßbild des Augenhintergrundes bei den Haussäugetieren. Arch Tierheilkd 70:449–472

    Google Scholar 

  28. Muhfelder TW, Nimetz J, Kreutzer J, Bebe D, Ward PA, Rosenfeld S (1979) C5 chemotactic fragment induces leucocyte production of tissue factor activity. A link between complement and coagulation. J Clin Invest 63:147–150

    Google Scholar 

  29. Nickel R, Schummer A, Seiferle E (1976) Lehrbuch der Anatomie der Haustiere, Bd. III. Parey, Berlin Hamburg, pp 118–121

    Google Scholar 

  30. Paterson JW, Dollery CT, Ramalho PS, Kohner EM (1967) The effects of platelet aggregates on the retinal microcirculation. Bibl Anat 9:85–91

    Google Scholar 

  31. Purtscher O (1910) Noch unbekannte Befunde nach Schädel-trauma. Ber Dtsch Ophthalmol Ges 36:294–303

    Google Scholar 

  32. Purtscher O (1912) Angiopathia retinae traumatica. Lymphorrhagien des Augengrundes. Arch Ophthalmol 82:347–371

    Google Scholar 

  33. Purtscher O (1916) Zur Kenntnis der Angiopathia retinae traumatica. Klin Monatsbl Augenheilkd 56:244–247

    Google Scholar 

  34. Reinecke RO, Kuwabara T, Cogan DG, Weis DR (1962) Retinal vascular patterns. Arch Opthalmol 67:470–475

    Google Scholar 

  35. Ring HG, David ND (1969) Experimental air embolism. Studies with fluorescein cinematography. Arch Ophthalmol 81:830–836

    Google Scholar 

  36. Schroer H, Scheurer G, Behrens-Baumann W (1992) Vascular occlusion of the retina. An experimental model. II. Platelet aggregates. Graefe's Arch Clin Exp Ophthalmol 230

  37. Till TGO, Johnson KJ, Kunkel R, Ward PA (1982) Intravascular activation of complement and acute lung injury. Dependance of neutrophils and toxic oxygen metabolites. J Clin Invest 69:1126–1135

    Google Scholar 

  38. Tvedten HW, Till GO, Ward PA (1985) Mediators of lung injury in mice following systemic activation of complement. Am J Pathol 119:92–100

    Google Scholar 

  39. Ward PA, Till GO, Hatherell JR, Annsley TN, Kunkel RG (1985) Systemic complement activation, lung injury and products of lipid proxidation. J Clin Invest 76:517–527

    Google Scholar 

  40. Weinstein L (1982) Syndrome of hemolysis, elevated liver enzymes and low platelet count: a severe consequence of hypertension in pregnancy. Am J Obstet Gynecol 142:159–167

    Google Scholar 

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Authors and Affiliations

  1. Augenklinik, Universität Göttingen, Robert-Koch-Strasse 40, W-3400, Göttingen, Federal Republic of Germany

    G. Scheurer, G. Praetorius & W. Behrens-Baumann

  2. Max-Planck-Institut für Experimentelle Medizin, Hermann-Rein-Strasse 3, W-3400, Göttingen, Federal Republic of Germany

    B. Damerau

Authors
  1. G. Scheurer
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  2. G. Praetorius
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  3. B. Damerau
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  4. W. Behrens-Baumann
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Additional information

This study was supported by a grant from the Deutsche Forschungsgemeinschaft (DFG; No. Be 1043)

Offprint requests to: W. Behrens-Baumann

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Scheurer, G., Praetorius, G., Damerau, B. et al. Vascular occlusion of the retina: an experimental model. Graefe's Arch Clin Exp Ophthalmol 230, 275–280 (1992). https://doi.org/10.1007/BF00176304

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  • DOI: https://doi.org/10.1007/BF00176304

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