Summary
Transmembrane Ca2+ currents were investigated by means of a whole-cell clamp technique in a hamster glucagon-secreting tumor cell line (ITC-1). Two types of Ca2+ current were identified in ITC-1 cells. The low-threshold and transient (T-type) current became detectable above the potential level around −60 mV and decayed rapidly with an inactivation time constant of 95 ms (at −40 mV and 23°C), while the high-threshold and long-lasting (L-type) one was activated by depolarization more positive to −30 mV with non-inactivating kinetics. The voltage dependence and kinetics of these currents were identical to those reported in guinea-pig pancreatic α2 cells. Both currents were augmented by equimolar substitution of Ca2+ with Ba2+ and completely abolished by adding 1 μM La3+. Phenytoin, a well known anti-epileptic drug and a postulated T-type specific Ca2+ current antagonist, surprisingly blocked the L-type current without affecting the T-type current in ITC-1 cells. While phenytoin antagonized the L-type Ba2+ current selectively, 60% of the current remained even in supramaximal concentration range over 500 μM. The residual component of the L-type current was completely abolished by adding nifedipine.
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Miyazaki, T., Hashiguchi, T., Hashiguchi, M. et al. Phenytoin partially antagonized L-type Ca2+ current in glucagon-secreting tumor cells (ITC-1). Naunyn-Schmiedeberg's Arch Pharmacol 345, 78–84 (1992). https://doi.org/10.1007/BF00175473
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DOI: https://doi.org/10.1007/BF00175473