Abstract
In order to understand the role of the medium osmolality on the metabolism of glumate-producing Corynebacterium glutamicum, effects of saline osmotic upshocks from 0.4 osnol. kg−1 to 2 osmol. kg−1 have been investigated on the growth kinetics and the intracellular content of the bacteria. Addition of a high concentration of NaCl after a few hours of batch culture results in a temporary interruption of the cellular growth. Cell growth resumes after about 1 h but at a specific rate that decreases with increasing medium osmolality. Investigation of the intracellular content showed, during the first 30 min following the shock, a rapid but transient influx of sodium ions. This was followed by a strong accumulation of proline, which rose from 5 to 110 mg/g dry weight at the end of the growth phase. A slight accumulation of intracellular glutamate from 60 to 75 mg/g dry weight was also observed. Accordingly, for Corynebacterium glutamicum an increased osmolality in the glutamate and proline synthesis during the growth phase.
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Guillouet, S., Engasser, J.M. Sodium and proline accumulation in Corynebacterium glutamicum as a response to an osmotic saline upshock. Appl Microbiol Biotechnol 43, 315–320 (1995). https://doi.org/10.1007/BF00172831
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DOI: https://doi.org/10.1007/BF00172831