Summary
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1.
The concentration of acrihellin rapidly declines in oxygenated Tyrode-solution, because the compound escapes from the organ-bath being enriched in droplets sprayed from the surface of the bubbled solution.
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2.
As checked by radiochromatography, acrihellin remains chemically unaltered during this process. Hellebrin and hellebrigenin persist in gassed Tyrode-solution, suggesting that the 3β-substituent dimethylacrylic acid endows acrihellin with amphiphilic properties, thus promoting its enrichment at gas-water interphases.
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3.
Measurements of the inotropic effects in guinea pig left atria performed at concentrations of acrihellin kept constant yielded a dose-response curve, which closely resembles that of the conventional cardioactive steroid ouabain.
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Herzig, S., Lüllmann, H., Mohr, K. et al. Acrihellin, a cardioactive steroid escaping from the organ-bath. Naunyn-Schmiedeberg's Arch Pharmacol 335, 326–330 (1987). https://doi.org/10.1007/BF00172805
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DOI: https://doi.org/10.1007/BF00172805