Summary
Olive mill waste-water was used for protein production in small-scale experiments, using non-sterilized medium without pH control. A 14 g/1 concentration of proteins, 61% chemical oxygen demand removal and a 58% reduction in total phenolic compounds were obtained using an Aspergillus niger strain. The removal of phenolic compounds resulted in a change in the colour of the waste-water from black to brown.
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References
Alazard D, Raimbault M (1981) Comparative study of amylolytic enzymes production by Aspergillus niger in liquid and solid-state fermentation. Appl Microbiol Biotechnol 12:113–117.
Balice V, Cera O (1984) Acidic phenolic fraction of the juice of olives determined by gas chromatographic method. Grasas Aceites 25:178–180.
Balice V, Boari G, Cera O, Abbaticchio P (1982) Indagine analitica sulle acque di vegetazione. Nota 1. Inquinamento 7:49–53.
Balice V, Carrieri C, Cera O, Rindone B (1988) The rate of tannin-like compounds from olive mill effluents in biological treatments. In: Hall ER, Hobson PN (eds) Proceedings of the Fifth International Symposium on Anaerobic Digestion, Bologna, Italy. Pergamon Press, Oxford, pp 275–280.
Blakebrough N, McManamey W, Tart KR (1978) Rheological measurements on Aspergillus niger fermentation systems. J Appl Chem Biotechnol 28:453–461.
Blom H, Thomassen MS (1985) Kinetic studies on strawberry anthocyanin hydrolysis by a thermostable anthocyanin-\-glucosidase from Aspergillus niger. Food Chem 17:157–168.
Boari G, Brunetti A, Passino R, Rozi A (1984) Anaerobic digestion of olive oil mill wastewaters. Agric Wastes 10:161–175.
Cichelli A, Solinas M (1984) I composti fenolici delle olive e dell'oloi di oliva. Riv Merceol 23:55–69.
Fernandez Diez MJ (1983) Olives. In: Rehm HJ, Reed G (eds) Biotechnology, vol. 5. Verlag Chemie, Weinheim, pp 379–397.
Fiesta Ros de Ursinos JA (1966) Installacion comercial para la obtencion de levura duras-pienso. Grasas Aceites 17:41–47.
Fiestas Ros de Ursinos JA (1981) Différentes utilisations des margines. In: F. A. O. (ed) Proc of Séminaire International sur la Valorisation des Sous-Produits de l'Olivier, Monastir, Tunisie, décembre 1981, pp 93–110.
Garcia JL, Guyot LP, Ollivier B, Trad M, Paycheng C (1982) Ecologie microbienne de la digestion anaérobie: technique de numération et d'isolement. Cah ORSTOM Sér Biol 45:3–15.
Giulietti AM, Ercoli E, Ertola R (1984) Purification and utilization of olive black water and distillery slops by microbial treatment. Acta Cient Venez 35:76–86.
Ikeda Y, Takahashi E, Yokogama K, Yoshimora Y (1972) Screening for microorganisms producing gallic acid from chinese and tara tannins. J Ferment Technol 50:361–370.
Kieslich K (1976) Microbiol transformations of non-steroid cyclic compounds. Wiley, New York.
Knechtel RJ (1978) A more economical method for the determination of chemical oxygen demand. Water Pollut Control May/June:25–29.
Makasinova SY, Martakov AA (1982) Assimilation of phenolic compounds by fungi of the genus Aspergillus. Tr In-st Mikrobiol Virusol Acad Nauk Kaz ssr 27:30–36.
Nelson N (1944) Photometric adaptation of Somogyi method for determination of glucose. J Biol Chem 153:375–380.
Raimbault M, Alazard D (1980) Culture method to study fungal growth in solid fermentation. Eur J Appl Microbiol Biotechnol 9:199–209.
Seng JM (1988) Chitine, chitosane et dérivés: de nouvelles perspectives pour l'industrie. Biofutur 71:40–44.
Somogyi M (1945) Determination of blood sugar. J Biol Chem 160:61–68.
Tanchev S, Joncheva N, Genov N, Codounis M (1980) Identification of anthocyanins contained in olives. Georgike Eurena 4:5–13.
Vazequez RA, Maestro DR, Graciani CE (1974) Componentes fenolicos de la aceituna. II. Poliphenoles del alpechin. Grasas Aceites 25:341–345.
Werner W, Rey HG, Wielinger RH (1977) Über die Eigenschaften eines neuen Chromogens für die Blutzucker Bestimmung nach der GOD/POD Methode. Z Anal Biochem 252:224–228.
Wood PJ, Siddiqui IR (1971) Determination of methanol and its application to measurements of pectin ester contents and pectin methyl esterase activity. Anal Biochem 39:418–428.
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Hamdi, M., Khadir, A. & Garcia, JL. The use of Aspergillus niger for the bioconversion of olive mill waste-waters. Appl Microbiol Biotechnol 34, 828–831 (1991). https://doi.org/10.1007/BF00169359
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DOI: https://doi.org/10.1007/BF00169359