Summary
Anaerobic growth of Escherichia coli was studied with glycerol as electron source and a three-electrode poised-potential system with potassium ferricyanide as nediator. Similar to fumarate, potassium ferricyanide was used as electron acceptor in batch-culture experiments. In experiments with regulated electrodes, glycerol was degraded completely, and an electron flow of 3.0 to 4.0 mA was obtained. The electron balances were in the range of 98%–107%, and a growth rate of 0.095 h−1 was calculated. These results are discussed with respect to the energetics of iron reduction by E. coli and by other bacteria.
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Emde, R., Swain, A. & Schink, B. Anaerobic oxidation of glycerol by Escherichia coli in an amperometric poised-potential culture system. Appl Microbiol Biotechnol 32, 170–175 (1989). https://doi.org/10.1007/BF00165883
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DOI: https://doi.org/10.1007/BF00165883