Summary
The effect of the calcium-specific chelant ethylene glycol-bis(\-aminoethyl ether)-N,N-tetraacetic acid (EGTA) on methanogenic granular sludge from a laboratory-scale upflow anaerobic sludge-blanket (UASB) reactor fed propionate and from a full-scale reactor treating paper-mill waste-water was studied. Upon treatment with EGTA both sludge types showed a decrease in the calcium and phosphorus content and a release of protein and polysaccharides, leading to a decrease in strength of papermill granular sludge and a disintegration of propionate-grown granules. After treatment of propionate-grown granular sludge with high EGTA concentrations, the methanogenic activity with propionate and acetate as test substrates decreased by 88 and 33%, respectively. The marked reduction in propionate oxidation activity may be caused by a disruption of the special juxtapositioning of bacteria in the granules.
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Grotenhuis, J.T.C., van Lier, J.B., Plugge, C.M. et al. Effect of ethylene glycol-bis(β-aminoethyl ether)-N,N-tetraacetic acid (EGTA) on stability and activity of methanogenic granular sludge. Appl Microbiol Biotechnol 36, 109–114 (1991). https://doi.org/10.1007/BF00164709
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DOI: https://doi.org/10.1007/BF00164709