Summary
An analysis of 4680 codons expressed by pathogenic Entamoeba histolytica showed the A+U content of coding sequences to be 67%. The preference for A+U resulted in an unusual codon usage with an A+U content of 84% in the third codon position. The data show a remarkable similarity to those obtained for Plasmodium falciparum.
This is a preview of subscription content, log in via an institution to check access.
References
Bulmer M (1987) Coevolution of codon usage and transfer RNA abundance. Nature 325:728–730
De Meester F, Bracha R, Huber M, Keren Z, Rozenblatt S, Mirelman D (1991) Cloning and characterization of an unusual elongation factor-lα cDNA from Entamoeba histolytica. Mol Biochem Parasitol 44:23–32
Edman U, Meza I, Agabian N (1987) Genomic and cDNA actin sequences from a virulent strain of Entamoeba histolytica. Proc Natl Acad Sci USA 84:3024–3028
Edman U, Meraz MA, Rausser S, Agabian N, Meza I (1990) Characterization of an immuno-dominant variable surface antigen from pathogenic and nonpathogenic Entamoeba histolytica. J Exp Med 172:879–888
Gouy M, Gautier C (1982) Codon usage in bacteria: correlation with gene expressivity. Nucleic Acids Res 10:7055–7074
Huber M, Garfinkel L, Gitler C, Mirelman D, Revel M, Rozenblatt S (1988) Nucleotide sequence analysis ofan Entamoeba histolytica ferredoxin gene. Mol Biochem Parasitol 31: 27–34
Makoff AJ, Oxer MD, Romanos MA, Fairweather NF, Ballantine S (1989) Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons. Nucleic Acids Res 17:10191–10202
Saul A, Battistutta D (1988) Codon usage in Plasmodium falciparum. Mol Biochem Parasitol 27:35–42
Stanley SL, Becker A, Kunz-Jenkins C, Foster L, Li E (1990) Cloning and expression of a membrane antigen of Entamoeba histolytica possessing multiple tandem repeats. Proc Natl Acad Sci USA 87:4976–4980
Tannich E, Horstmann RD, Knobloch J, Arnold HH (1989) Genomic DNA differences between pathogenic and nonpathogenic Entamoeba histolytica. Proc Natl Acad Sci USA 86: 5228–5122
Tannich E, Bruchhaus I, Walter RD, Horstmann RD (1991a) Pathogenic and nonpathogenic Entamoeba histolytica: identification and molecular cloning of an iron-containing superoxide dismutase. Mol Biochem Parasitol 49:61–72
Tannich E, Ebert F, Horstmann RD (1991b) Primary structure of the 170-kDa surface lectin of pathogenic Entamoeba histolytica. Proc Natl Acad Sci USA 88:1849–1853
Tannich E, Scholze H, Nickel R, Horstmann RD (1991c) Homologous cysteine proteinases of pathogenic and nonpathogenic Entamoeba histolytica: differences in structure and expression. J Biol Chem 266:4798–4803
Torian BE, Flores BM, Stroeher VL, Hagen FS, Stamm WE (1990) cDNA sequence analysis of a 29-kDA cysteine-rich surface antigen of pathogenic Entamoeba histolytica. Proc Natl Acad Sci USA 87:6358–6362
Wada K, Wada Y, Doi H, Ishibashi F, Gojobori T, Ikemura, T (1991) Codon usage tabulated from the GenBank genetic sequence data. Nucleic Acids Res 19:s1981-s1986
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tannich, E., Horstmann, R.D. Codon Usage in Pathogenic Entamoeba histolytica . J Mol Evol 34, 272–273 (1992). https://doi.org/10.1007/BF00162976
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00162976