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Influence of broth dilution on the disruption of Escherichia coli

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Biotechnology Techniques

Summary

The effect of cell concentration (5 to 150 g/L wet wt after broth dilution) on homogenizer disruption efficiency and homogenate viscosity is reported for E. coli. Broth dilution increases homogenizer efficiency and decreases feed and homogenate viscosity. However, this increase in disruption efficiency is not sufficient to warrant dilution of the broth prior to homogenization. The optimal feed concentration is the maximum possible that does not lead to practical handling difficulties due to high viscosity.

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Authors and Affiliations

  1. Department of Chemical Engineering, The University of Adelaide, 5005, Adelaide, Australia

    A. R. Kleinig, C. J. Mansell, Q. D. Nguyen, A. Badalyan & A. P. J. Middelberg

  2. Co-operative Research Centre for Tissue Growth and Repair, Australia

    A. R. Kleinig, C. J. Mansell, Q. D. Nguyen, A. Badalyan & A. P. J. Middelberg

Authors
  1. A. R. Kleinig
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  2. C. J. Mansell
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  3. Q. D. Nguyen
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  4. A. Badalyan
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  5. A. P. J. Middelberg
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Kleinig, A.R., Mansell, C.J., Nguyen, Q.D. et al. Influence of broth dilution on the disruption of Escherichia coli . Biotechnol Tech 9, 759–762 (1995). https://doi.org/10.1007/BF00159244

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  • DOI: https://doi.org/10.1007/BF00159244

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