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The effect of homogeniser impact distance on the disruption of Escherichia coli

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Biotechnology Techniques

Summary

The effect of homogeniser impact distance on the disruption efficiency of stationary and growth-phase Escherichia coli is examined. Disruption efficiency decreases as impact distance increases as seen previously for yeasts. However, the dependence on impact distance is considerably less than previously reported for yeast. Results suggest that homogeniser performance can be improved by decreasing impact distance. This effect is modelled.

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References

  • Gray, P.P., Dunnill, P., Lilly, M.D. (1972). Proc. IV IFS: Ferment. Technol. Today, Terui, E., Ed., pp 347–351.

  • Hetherington, P.J., Follows, M., Dunnill, P., Lilly, M.D. (1971). Trans. IChemE., 49, 142–148.

    Google Scholar 

  • Hopkins, T.R. (1991). In Purification and analysis of recombinant proteins, Seetharam, R. and Sharma, S.K., Eds., Marcel Dekker (New York), pp 57–83.

    Google Scholar 

  • Kelemen, M.V.; Sharpe, J.E.E. (1979). J. Cell. Sci., 35, 431–441.

    Google Scholar 

  • Keshavarz Moore, E.; Hoare, M.; Dunnill, P. (1990). Enzyme Microb. Technol., 12, 764–770.

    Google Scholar 

  • Kleinig, A.R. and Middelberg, A.P.J. (1994). In Better living through innovative biochemical Engineering, Teo, W.K., Yap, M.G.S. and Oh, S.K.W. Eds., pp 607–609.

  • Middelberg, A.P.J. (1992). PhD Thesis, The University of Adelaide, Australia.

    Google Scholar 

  • Middelberg, A.P.J., O'Neill, B.K., Thomas, C.J. (1994). Biotechnol. Prog., 10, 109–113.

    Google Scholar 

  • Middelberg, A.P.J., O'Neill, B.K., Bogle, I.D.L. (1992a). Trans. IChemE., 70, 205–212.

    Google Scholar 

  • Middelberg, A.P.J., O'Neill, B.K., Bogle, I.D.L., Gully, N.J., Rogers, A.H., Thomas, C.J. (1992b). Trans. IChemE., 70, 213–218.

    Google Scholar 

  • Phipps, L.W. (1975). J. Phys. D: Appl. Phys., 8, 448–462.

    Google Scholar 

  • Sauer, T., Robinson, C.W., Glick, B.R. (1989). Biotechnol. Bioeng., 33, 1330–1342.

    Google Scholar 

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Kleinig, A.R., O'Neill, B.K. & Middelberg, A.P.J. The effect of homogeniser impact distance on the disruption of Escherichia coli . Biotechnol Tech 10, 199–204 (1996). https://doi.org/10.1007/BF00158946

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  • DOI: https://doi.org/10.1007/BF00158946

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