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Cloning, sequencing and expression of the amylase isozyme gene from Pseudomonas sp. KFCC 10818

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Summary

A gene coding for amylase was cloned and sequenced from an alkalophilic Pseudomonas sp. KFCC 10818. The coding region for the amylase precursor contained 1,692 nucleotides. The presumed Shine-Dalgarno sequence, AAGG, was located at 8 nucleotides upstream from the ATG initiation codon. The precursor protein had a putative signal peptide of 25 amino acid residues at its amino terminus. The Pseudomonas amylase had four highly conserved regions as other α-amylases. The amylase expressed from E. coli harboring the Pseudomonas gene produced maltose and maltotriose from soluble starch.

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Authors and Affiliations

  1. Dept. of Biology, Chonnam National University, 500-757, Kwangju, Korea

    Eun-Seon Kim, Hye-Kyung Na, Deok-Young Jhon, Ook Joon Yoo, Soon-Bae Chun & In-Sun Wui

  2. Dept. of Food and Nutrition, Chonnam National University, 500-757, Kwangju, Korea

    Hye-Kyung Na & Deok-Young Jhon

  3. Dept. of Microbiology, Chonnam National University, 500-757, Kwangju, Korea

    Soon-Bae Chun

  4. Dept. of Biological Science, KAIST, 305-701, Taejon, Republic of Korea

    Ook Joon Yoo

Authors
  1. Eun-Seon Kim
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  2. Hye-Kyung Na
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  3. Deok-Young Jhon
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  4. Ook Joon Yoo
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  5. Soon-Bae Chun
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  6. In-Sun Wui
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Additional information

The nucleotide sequence reported in this paper has been submitted in the GenBank/EMBL database with accession number(s) U40056.

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Kim, ES., Na, HK., Jhon, DY. et al. Cloning, sequencing and expression of the amylase isozyme gene from Pseudomonas sp. KFCC 10818. Biotechnol Lett 18, 169–174 (1996). https://doi.org/10.1007/BF00128674

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