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Kinetics of aerobic biodegradation of benzene and toluene in sandy aquifer material

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Abstract

Monod's equation adequately described aerobic biodegradation rates of benzene and toluene by the microbial population of a sandy aquifer when these compounds were initially present at concentrations lower than 100 mg/l each. Concentrations higher than 100 mg/l were inhibitory, and no benzene or toluene degradation was observed when these compounds were initially present at 250 mg/l each. The Monod coefficients were calculated as k = 8.3 g-benzene/g-cells/day and Ks = 12.2 mg/l for benzene, and k = 9.9 g-toluene/g-cells/day and Ks = 17.4 mg/l for toluene. Specific first-order coefficients would be 0.68 l/mg.day for benzene and 0.57 l.mg.day for toluene.

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References

  • Alexander M (1977) Introduction to Soil Microbiology, 2nd edition. John Wiley & Sons, NY

    Google Scholar 

  • Alexander M & Scow KM (1989) Kinetics of Biodegradation in Soil. In: Shawney BL & Browns K (Eds) Reactions and Movement of Organic Chemicals in Soils. Soil Science Society of America 22: 243–269

  • Barker JF, Patrick GC & Major D (1987) Natural Attenuation of Aromatic Hydrocarbons in a Shallow Sand Aquifer. Groundwater Monitoring Review (Winter): 64–71

  • Borden RC & Bedient PB (1986) Transport of Dissolved Hydrocarbons Influenced by Oxygen-Limited Biodegradation 1. Theoretical Development. Water Resources Research 22: 1973–1982

    Google Scholar 

  • Button DK (1985) Kinetics of Nutrient-Limited Transport and Microbial Growth. Microbiological Reviews 49: 270–297

    Google Scholar 

  • Button DK & Robertson BR (1986) Dissolved Hydrocarbon Metabolism: The Concentration-Dependent Kinetics of Toluene Oxidation in Some North American Estuaries. Limnol. Oceanogr. 31: 101–111

    Google Scholar 

  • Chiang CY, Salanitro JP, Chai EY, Colthart JD & Klein CL (1989) Aerobic Biodegradation of Benzene, Toluene, and Xylene in a Sandy Aquifer — Data Analysis and Computer Modeling. Groundwater 27: 823–834

    Google Scholar 

  • Davis E, Murray HE, Liehr JG & Powers EL (1981) Basic Microbial Degradation Rates and Chemical Byproducts of Selected Organic Compounds. Water Research 15: 1125–1127

    Google Scholar 

  • Dowd JE & Riggs DS (1965) A Comparison of Estimates of Michaelis-Menten Kinetic Constants from Various Linear Transformations. The Journal of Biological Chemistry 240: 863–869

    Google Scholar 

  • Grady CP & Lim HC (1980) Biological Wastewater Treatment-Theory and Applications. Marcel Dekker, Inc. New York, NY

    Google Scholar 

  • Grady CP, Aichinger G, Cooper SF & Naziruddin M (1989) Biodegradation Kinetics for Selected Toxic/Hazardous Compounds. Proceedings of the 1989 A&WMA/EPA International Symposium on Hazardous Waste Treatment: Biosystems for Pollution Control. Cincinnati, OH (February 1989): 141–153

  • Harvey RW, Smith RL & George L (1984) Effect of Organic Contamination upon Microbial Distributions and Heterotrophic Uptake in Cape Cod, Mass., Aquifer. Appl. Environ. Microbiol. 48: 1197–1202

    Google Scholar 

  • Hutchins SR, Wilson JT, Douglas RH & Hendrix DJ (1990) Field and Laboratory Evaluation of the Use of Nitrate to Remove BTX from a Fuel Spill. Proceedings: Environmental Research Conference on Groundwater Quality and Waste Disposal. Washington D.C. May 2–4, 1989. Edited by I.P. Muraka, Electric Power Research Institute, Palo Alto, CA. and S. Cordle, U.S. Environmental Protection Agency. Washington, DC (pp 29–1–29–17)

    Google Scholar 

  • Johnson PC, Kemblowski MW & Colthart JD (1990) Quantitative Analysis for the Cleanup of Hydrocarbon-Contaminated Soils by In-Situ Soil Venting. Groundwater 28: 413–429

    Google Scholar 

  • Jones BE (1984) Fate of Toxic Organic Compounds in Activated Sludge and Integrated Activated Sludge/Carbon Treatment Systems. Ph.D. thesis, Environmental and Water Resources Engineering, Department of Civil Engineering. The University of Michigan, Ann Arbor

  • Jørgensen C, Flyvberg J, Jensen BK, Arvin E, Olsen SK & Mortensen E (1990) Toluene Metabolism and its effects on o-Cresol Transformation under Nitrate Reducing Conditions. COST Seminar on Anaerobic Biodegradation of Xenobiotic Compounds, Copenhagen, November 1990

  • Kukor JJ & Olsen RH (1989) Diversity of Toluene Degradation Following Long Term Exposure to BTEX in situ. In: Kamely D, Chakabarty A & Omenn G (Eds) Biotechnology and Biodegradation—1989 (pp 5–21)

  • Lawrence A & McCarty PL (1970) Unified Basis for Biological Treatment Design and Operation. Journal of the Sanitary Engineering Division. ASCE 96: 757–778

    Google Scholar 

  • Lee MD, Thomas JM, Borden RC, Bedient PB, Ward CH & Wilson JT (1988) Biorestoration of aquifers contaminated with organic compounds. CRC Critical Reviews in Environmental Control. 1: 29–89

    Google Scholar 

  • MacQuarrie KTB, Sudicky EA & Frind EO (1990) Simulation of Biodegradable Organic Contaminants in Groundwater 1. Numerical Formulation in Principal Directions. Water Resources Research 26: 207–222

    Google Scholar 

  • Molz F, Widdowson J, Benefield MA et al. (1986) Simulation of Microbial Growth Dynamics Coupled to Nutrient and Oxygen Transport in Porous Media. Water Resour. Res. 22: 1207–1216

    Google Scholar 

  • Monod J (1949) The Growth of Bacterial Cultures. Annual Review of Microbiology 3: 371–394

    Google Scholar 

  • Robertson BR & Button DK (1987) Toluene Induction and Uptake Kinetics and Their Inclusion in the Specific-Affinity Relationships Describing Rates of Hydrocarbon Metabolism. Appl. Environ. Microbiol. 53: 2193–2205

    Google Scholar 

  • Sheehan PJ, Schneiter RW, Mohr TKG & Gersberg RM (1988) Bioreclamation of Gasoline Contamination Without Oxygen Addition. In: Proceedings of the Second National Outdoor Action Conference on Aquifer Restoration, Groundwater Monitoring, and Geophysical Methods, Vol 1. NWWA, Las Vegas, Nevada, May 23–26, 1988 (pp 183–199)

    Google Scholar 

  • Shimp R & Pfaender F (1985) Influence of Easily Degradable Naturally Occurring Carbon Substrates on Biodegradation of Monosubstituted Phenols by Aquatic Bacteria. Appl. Environ. Microbiol. 49: 394–401

    Google Scholar 

  • Slater JH & Lovatt D (1984) Biodegradation and the Significance of Microbial Communities. In: Gibson DT (Ed) Microbial Degradation of Organic Pollutants (pp 439–485). Marcel Dekker, Inc. NY

    Google Scholar 

  • Thomas JM, Gordy VR, Fiorenza S & Ward CH (1990) Biodegradation of BTEX in Subsurface Materials Contaminated with Gasoline: Granger, Indiana. Water Sci. Tech. 6: 53–62

    Google Scholar 

  • Tiedje JM (1987) Ecology of Denitrification and Dissimilatory Nitrate Reduction to Ammonium. In: Zehnder AJB (Ed) Environmental Microbiology of Anaerobes (pp 179–244). John Wiley & Sons, NY

    Google Scholar 

  • Verheul JHAM, van den Berg R & Eikelboom DH (1988) In situ Biorestoration of a Subsoil Contaminated with Gasoline. In: Wolf K, van den Brink WJ & Colon FJ (Eds) Contaminated Soil '88 (pp 705–716). Kluwer Academic Publishers

  • Vecht SE, Platt MW, Er-El Z & Goldberg I (1988) The growth of Pseudomonas putida on m-Toluic Acid and toluene in batch and in Chemostat Cultures. Appl. Microbiol. Biotechnol. 27: 587–592

    Google Scholar 

  • Webster JJ, Hampton GJ, Wilson JT, Ghiorse WC & Leach FR (1985) Determination of Cell Numbers in Subsurface Samples. Groundwater 23: 17–23

    Google Scholar 

  • Werner P (1985) A New Way for the Decontamination of Polluted Aquifers by Biodegradation. Water Supply 3: 41–47

    Google Scholar 

  • Widdowson MA, Molz FJ & Benefield LD (1988) A numerical Transport Model for Oxygen- and Nitrate-Based Respiration Linked to Substrate and Nutrient Availability in Porous Media. Water Resour. Res. 24: 1553–1565

    Google Scholar 

  • Wilson J, Leach L, Michalowski J, Vandergrift S & Callaway R (1990) In situ Reclamation of Spills from Underground Storage Tanks: New Approaches for Site Characterization, Project Design, and Evaluation of Performance. Proceedings: Environmental Research Conference on Groundwater Quality and Waste Disposal. Washington D.C. May 2–4, 1989. Edited by I.P. Muraka, Electric Power Research Institute, Palo Alto, CA. and S. Cordle, U.S. Environmental Protection Agency. Washington DC (pp 30–1–30–16)

    Google Scholar 

  • Zoetman BCJ, De Greef E & Brikmann FJJ (1981) Persistency of Organic Chemicals in Groundwater, Lessons from Soil Pollution Incidents in the Netherlands. Sci. Tot. Environ. 21: 187–202

    Google Scholar 

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Alvarez, P.J.J., Anid, P.J. & Vogel, T.M. Kinetics of aerobic biodegradation of benzene and toluene in sandy aquifer material. Biodegradation 2, 43–51 (1991). https://doi.org/10.1007/BF00122424

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