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Stably transformed cell lines from protoplasts of maize endosperm suspension cultures

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Abstract

Protoplasts were isolated from Zea mays (L.) A69Y endosperm suspension cultures and transformed by polyethylene glycol mediated DNA uptake with chimaeric gene constructs containing β-glucuronidase (GUS) or neomycin phosphotransferase II (NPTII); GUS-expressing and Kanamycin-resistant cultures were recovered. The transformed cells showed integration of the introduced foreign genes into genomic DNA and maintained their ability to synthesize endosperm-specific reserve proteins (zeins). No deletion or rearrangement of zein genes were observed in transformed cultures. Stable transformation of cultured maize endosperm cells may therefore represent a new methodological approach for the study of the transcriptional regulation of endosperm-expressed genes.

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Abbreviations

BMS:

Black mexican sweet

FDA:

Fluorescine-diacetate

GUS:

β-glucuronidase

MUG:

4-methylumbelliferyl-β-d-glucuronide

NPTII:

neomycin phosphotransferase

PEG:

polyethylene glycol

X-GLUC:

5-bromo-4-chloro-3-indolyl-β-d-glucuronide

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Authors and Affiliations

  1. Istituto Biosintesi Vegetali, C.N.R., Via Bassini, 15-20133, Milano, Italy

    Sara Faranda, Annamaria Genga, Angelo Viotti & Lucia A. Manzocchi

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  1. Sara Faranda
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  2. Annamaria Genga
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  3. Angelo Viotti
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  4. Lucia A. Manzocchi
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Faranda, S., Genga, A., Viotti, A. et al. Stably transformed cell lines from protoplasts of maize endosperm suspension cultures. Plant Cell Tiss Organ Cult 37, 39–46 (1994). https://doi.org/10.1007/BF00048115

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  • DOI: https://doi.org/10.1007/BF00048115

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