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Protoplast culture and plant regeneration in Lycium barbarum L.

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Abstract

Protoplasts were isolated from leaves of the woody plant Lycium barbarum L. and cultured in liquid nutrient medium TM-2 at a density of 104–105 cells ml-1. After ten days of culture, regenerated colonies were transferred to the agar-solidified medium TM-3, and 5–7 days later to regeneration media PRM or TM-4. Formation of shoots was observed after 30–40 days. Completely formed and rooted plants were transferred to the soil. Cytological and morphological analysis of the regenerated plants revealed relative genetic stability of this species in the process ‘protoplast — plant’. The results obtained allow us to conclude that L. barbarum can be used in the experiments on somatic hybridization or direct gene transfer.

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Authors and Affiliations

  1. N.G. Kholodny Institute of Botany, Ukrainian SSR Academy of Sciences, Repina, 2, 252601, Kiev-GSP-1, USSR

    Yakov I. Ratushnyak, Nickolai M. Piven & Vladimir A. Rudas

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  1. Yakov I. Ratushnyak
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  2. Nickolai M. Piven
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  3. Vladimir A. Rudas
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Ratushnyak, Y.I., Piven, N.M. & Rudas, V.A. Protoplast culture and plant regeneration in Lycium barbarum L.. Plant Cell Tiss Organ Cult 17, 183–190 (1989). https://doi.org/10.1007/BF00046866

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  • DOI: https://doi.org/10.1007/BF00046866

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