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Molecular cloning of an alanine aminotransferase from NAD-malic enzyme type C4 plant Panicum miliaceum

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Abstract

We have determined the nucleotide sequence of a cDNA encoding AlaAT-2, which is believed to function in the C4-pathway of Panicum miliaceum. An open reading frame (1446 bp) encodes a protein of 482 amino acid residues. The deduced amino acid sequence of AlaAT-2 shows 44.2 and 44.8% homology with the amino acid sequences of AlaATs from rat and human livers, respectively. Northern blot analysis showed that the gene encoding AlaAT-2 in mesophyll and bundle sheath cells was the same and transcribed similarly in the cells. The level of translatable mRNA for AlaAT-2 increased dramatically during greening.

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  1. Department of Agricultural Chemistry, School of Agriculture, Nagoya University, Chikusa, 464-01, Nagoya, Japan

    Daeyoung Son & Tatsuo Sugiyama

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  1. Daeyoung Son
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  2. Tatsuo Sugiyama
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Son, D., Sugiyama, T. Molecular cloning of an alanine aminotransferase from NAD-malic enzyme type C4 plant Panicum miliaceum . Plant Mol Biol 20, 705–713 (1992). https://doi.org/10.1007/BF00046455

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  • DOI: https://doi.org/10.1007/BF00046455

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