Abstract
Embryogenic calluses of sugarcane capable of regenerating green plants after long-term culture were sought. The largest quantities of embryogenic calluses were produced on Murashige & Skoog medium, but cultures maintained on Chu N6 medium remained embryogenic and totipotent longer. Both media contained 4.5 μM 2,4-dichlorophenoxyacetic acid (2,4-d). The effect of supplements on somatic embryogenesis was examined. Kinetin (0.5 μM) and 10% (v/v) coconut water in callus initiation medium were inhibitory to subsequent embryogenesis. Embryogenic calluses on N6 medium increased in fresh weight with proline concentration up to 90 mM. Maximum fresh weight was achieved with 5% sucrose. Although genotypic differences were observed, embryogenesis occurred in all 17 sugarcane clones tested. Embryogenic calluses of one cultivar regenerated green plants after 16 months, but suspensions were totipotent for only 8 months. Total number of regenerated plants decreased with time in culture, while the number of pale green plants increased starting after 5 months in culture.
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References
Ahloowahlia BS & Maretzki A (1983) Plant regeneration via somatic embryogenesis in sugarcane. Plant Cell Rep. 2: 21–25
Ahn BJ, Huang FH & King JW (1985) Plant regeneration through somatic embryogenesis in common bermudagrass tissue culture. Crop Sci. 25: 1107–1109
Ammirato PV (1983) Embryogenesis. In: Evans DA, Sharp WR & Yamada Y (Eds) Handbook of Plant Cell Culture, Vol 1 (pp 82–123) MacMillan, New York
Armstrong CL & Green CE (1985) Establishment and maintenance of friable, embryogenic maize callus and the involvement of l-proline. Planta 164: 207–214
Chu C-C, Wang C-C, Sun C-S, Hsu C, Yin K-C, Chu C-Y & Bi F-Y (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sin. 18: 659–668
Datta SK, Peterhans A, Datta K & Potrykus I (1990) Genetically engineered fertile indica-rice recovered from protoplasts. Bio Technology 8: 736–740
Fitch MMM & Moore PH (1990) Comparison of 2,4-d and picloram for selection of long-term totipotent green callus of sugarcane. Plant Cell Tiss. Org. Cult. 20: 157–163
Fromm ME, Morrish F, Armstrong C, Williams R, Thomas J & Klein TM (1990) Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technology 8: 833–839
Gordon-Kamm WJ, Spencer TM, Mangano ML, Adams TR, Daines RJ, Start WG, O'Brien JV, Chambers SA, Adams WR, Willetts NG, Rice TB, Mackey CJ, Krueger RW, Kausch AP & Lemaux PG (1990) Transformation of maize cells and regeneration of fertile transgenic plants. The Plant Cell 2: 603–618
Green CE & Phillips RL (1975) Plant regeneration from tissue cultures of maize. Crop Sci. 15: 417–421
Guiderdoni E (1986a) L'embryogenèse somatique des explants foliaires de canne à sucre (Saccharum sp.) cultivés in vitro. II initiation des cultures. Agron. Trop. 41: 50–59
Guiderdoni E (1986b) L'embryogenèse somatique des explants foliaires de canne à sucre (Saccharum sp.) cultivés in vitro I-étude anatomique de la morphogenese. Agron. Trop. 41: 160–166
Guiderdoni E & Demarly Y (1988) Histology of somatic embryogenesis in cultured leaf segments of sugarcane plantlets. Plant Cell Tiss. Org. Cult. 14: 71–88
Ho WJ & Vasil IK (1983a) Somatic embryogenesis in sugar cane (Saccharum officinarum L.). 1. The morphology and physiology of callus formation and the ontogeny of somatic embryos. Protoplasma 118: 169–180
Ho WJ & Vasil IK (1983b) Somatic embryogenesis in sugar cane (Saccharum officinarum L.). 2. Growth and plant regeneration from embryogenic cell suspension cultures. Ann. Bot. 51: 719–726
Kamo KK & Hodges TK (1986) Establishment and characterization of long-term embryogenic maize callus and cell suspension cultures. Plant Sci. 45: 111–117
Liu M-C & Chen W-H (1974) Histological studies on the origin and process of plantlet differentiation in sugarcane callus mass. International Society of Sugarcane Technologists-Proceedings, 15th (pp 118–128). Durban, South Africa
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nadar HM, Soepraptopo S, Heinz DJ & Ladd SL (1978) Fine structure of sugarcane (Saccharum sp.) callus and the role of auxin in embryogenesis. Crop Sci. 18: 210–216
Prioli LM & Sondahl MR (1989) Plant regeneration and recovery of fertile plants from protoplasts of maize (Zea mays L.). Bio/Technology 7: 589–594
Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D & Detmer JJ (1988) Genetically transformed maize plants from protoplasts. Science 240: 204–207
Srinivasan C & Vasil IK (1986) Plant regeneration from protoplasts of sugarcane (Saccharum officinarum L.). J. Plant. Physiol. 126: 41–48
Thomas E, King PJ & Potrykus I (1979) Improvement of crop plants via single cells in vitro-an assessment. Z. Pflanzenzüchtg. 82: 1–30
Vasil IK (1983) Regeneration of plants from single cells of cereals and grasses. In: Lurquin PF & Kleinhofs A (Eds) Genetic Engineering in Eukaryotes (pp 233–252) Plenum Publishing Corp., New York
Williams EG & Maheswaran G (1986) Somatic embryogenesis: factors influencing coordinated behavior of cells as an embryogenic group. Ann. Bot. 57: 443–462
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Published as Paper No. 785 in the journal series of the Experiment Station, HSPA
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Fitch, M.M.M., Moore, P.H. Long-term culture of embryogenic sugarcane callus. Plant Cell Tiss Organ Cult 32, 335–343 (1993). https://doi.org/10.1007/BF00042297
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DOI: https://doi.org/10.1007/BF00042297