Abstract
The development of haploid callus, embryos and plantlets from cultured anthers and the various factors affecting androgenesis in Peltophorum pterocarpum (Copper pod), a tropical legume tree is reported. A pretreatment of flower buds at moderate temperature of 14°C for 8 days was most effective for callus production. The colour of the anther was found to be a reliable and efficient indicator for identification of suitable stage of anther for culture. The frequency of anthers which produced callus and shoots was highest when anthers were cultured at mid or late-uninucleate stage. A high sucrose concentration of 10% is a specific media requirement for androgenesis. The haploid nature of the embryos, callus and regenerated plants (n=14) were confirmed by chromosome count.
Similar content being viewed by others
Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- KN:
-
kinetin
- NAA:
-
naphthaleneacetic acid
- BAP:
-
bezylaminopurine
References
Bajaj YPS (1983) In vitro production of haploid In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Techniques for propogation and breeding. Macmillan, New York Handbook of Plant cell culture, Vol. 1, pp. 228–287.
Bernardo FF (1965) Processing of Gossypium microspores for first division chromosomes. Stain Tech 40: 200–208.
Chaturvedi HC, Sharma AK (1985) Androgenesis in Citrus aurantifolia (Christn.) Swingle. Planta 165: 142–144.
Chen CH, Chen TF, Chien CF, Wang CH, Chang SJ, HsuHe, Du HH, Ho YT, Lu TM (1978) Induction of pollen plantlets of Hevea brasiliensis Muell.-Arg. Acta Genet Sin 5: 99–107.
Chen Z, Qian C, Qin M, Xu X, Xiao Y (1982) Recent advances in anther culture of Hevea brasiliensis Muell.-Arg. Theor Appl Genet 62: 103–108.
De DN, Lakshmana Rao PV (1983) Androgenetic haploid callus of tropical leguminous trees. In: Sen SK, Giles K (eds) Plant cell culture in crop improvement. Plenum Press, New York, pp. 469–474.
Gharyal PK, Maheswari SC (1983) In vitro differentiation of plantlets in anther cultures of Albizzia lebbek L. Plant cell Rep 2: 308–309.
Gharyal PK, Rashid A and Maheshwari SC (1983) Androgenic response from cultured anthers of a leguminous tree, Cassia siamea Lam. Protoplasma 118: 91–93.
Kao KN (1975) A chromosomal staining method for cultured cells In: Gamborg OL, Wetter RL (eds) Plant tissue culture methods. National Research Council of Canada, Prairie Regional Laboratory, Saskatoon, pp. 63–65.
Lakshmana Rao PV (1985) Induction and nutrition of diploid and haploid callus and plantlet regeneration of three tropical legume trees. Ph.D. Thesis, Indian Institute of Technology, Kharagpur.
Maheswari SC, Rashid A, Tyagi AK (1983) Haploids from pollen grains-prospect and retrospect. Am J Bot 69: 865–879.
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497.
Nair S, Gupta PK, Mascarenhas AF (1983) Haploid plants from in vitro anther culture of Annona squamosa Linn. Plant Cell Reports 2: 198–200.
Radojevic L (1978) In vitro induction of androgenic plantlets in Aesculus hippocastanus. Protoplasma 96: 369–374.
Sunderland N, Roberts M (1977) New approach to pollen culture. Nature 270: 236–238.
Thanh-Tuyen NT, DeGuzman EV (1983) Formation of pollen embryos in cultured anthers of coconut (Cocos nucifera L.) Plant Sci Lett 29: 81–88.
Wang CH-CH, Sun CHS, Chu ZCH (1975) The induction of Populus pollen plants. Acta Bot Sinica 17: 56–59.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rao, P.V.L., De Deepesh, N. Haploid plants from in vitro anther culture of the leguminous tree, Peltophorum pterocarpum (DC) K. Hayne (Copper pod). Plant Cell Tiss Organ Cult 11, 167–177 (1987). https://doi.org/10.1007/BF00040423
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00040423