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High capacity of plant regeneration from callus of interspecific hybrids with cultivated barley (Hordeum vulgare L.)

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Abstract

Callus was induced from hybrids between cultivated barley (Hordeum vulgare L. ssp. vulgare) and ten species of wild barley (Hordeum L.) as well as from one backcross line ((H. lechleri x H. vulgare) x H. vulgare). Successful callus induction and regeneration of plants were achieved from explants of young spikes on the barley medium J 25–8. The capacity for plant regeneration was dependent on the wild parental species. In particular, combinations with four related wild species, viz. H. jubatum, H. roshevitzii, H. lechleri, and H. procerum, regenerated high numbers of plants from calli.

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Jørgensen, R.B., Jensen, C.J., Andersen, B. et al. High capacity of plant regeneration from callus of interspecific hybrids with cultivated barley (Hordeum vulgare L.). Plant Cell Tiss Organ Cult 6, 199–207 (1986). https://doi.org/10.1007/BF00040005

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  • DOI: https://doi.org/10.1007/BF00040005

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