Skip to main content
SpringerLink
Log in

Application of isozyme electrophoresis for purity testing and cultivar identification of F1 hybrids of Brassica oleracea

  • Published:
Euphytica Aims and scope Submit manuscript

Summary

Six isozyme genes were analyzed in seed samples of 65 commercial F1 hybrids of four horticultural groups of Brassica oleracea (cabbage, Brussels sprouts, sprouting broccoli and cauliflower). Results obtained from electrophoretic assays led to the following conclusions: 1) the electrophoretic test of F1 hybrid purity was possible in 59 (91%) of the hybrids analyzed, since their inbred parents were apparently fixed each for a different allele in at least on of the loci studied; 2) forty-eight (74%) of the hybrids were individually distinguished by their isozyme phenotype; 3) high levels of segregation in the inbred parents were inferred from the analysis of a sample of seeds of each hybrid.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andersen, H. J., 1982. Isoenzyme characters of 47 barley cultivars and their application in cultivar identification. Seed Sci. Technol. 10: 405–413.

    Google Scholar 

  • Arús, P., 1983. Genetic purity of commercial seed lots. In: S. D. Tanksley & T. J. Orton (Eds), Isozymes in plant genetics and breeding. Elsevier Sci. Pub. Co. Amsterdam. pp. 415–423.

    Google Scholar 

  • Arús, P., 1984. Allozyme variability in Brassica oleracea and applications in plant breeding. Ph. D. Thesis. University of California, Davis.

  • Arús, P. & T. J. Orton, 1983. Inheritance and linkage relationships of isozyme loci in Brassica oleracea. J. Hered. 74: 405–412.

    Google Scholar 

  • Arús, P., S. D. Tanksley, T. J. Orton & R. A. Jones, 1982. Electrophoretic variation as a tool for determining seed purity and for breeding hybrid varieties of Brassica oleracea. Euphytica 31: 417–428.

    Google Scholar 

  • Bringhurst, R. S., S. Arulsekar, J. F. HannockJr & V. Voth, 1981. Electrophoretic characterization of strawberry cultivars. J. Amer. Soc. Hort. Sci. 106(5): 684–687.

    Google Scholar 

  • Cardy, B. J. & L. W. Kannenberg, 1982. Allozymic variability among maize inbred lines and hybrids: applications for cultivar identification. Crop. Sci. 22: 1016–1020.

    Google Scholar 

  • Samaniego, F. J. & P. Arús, 1983. On estimating the sib proportion in seed-purity determinations. Biometrics 39: 563–572.

    Google Scholar 

  • Stuber, C. W. & M. M. Goodman, 1983. Allozyme phenotypes for popular and historically important inbred lines of corn, Zea mays L. U. S. Agric. Res. Serv., ARR-S-16.

  • Torres, A. M. & M. M. Bergh, 1980. Fruit and leaf isozymes as genetic markers in avocado. J. Amer. Soc. Hort. Sci. 105 (4): 614–619.

    Google Scholar 

  • Wendel, J. F. & C. R. Parks, 1983. Cultivar identification in Camellia japonica L. using allozyme polymorphisms. J. Amer. Soc. Hort. Sci. 108 (2): 290–295.

    Google Scholar 

  • Weeden, N. F., 1984. Distinguishing among white seeded bean cultivars by means of allozyme genoypes. Euphytica 33: 199–208.

    Google Scholar 

  • Wills, A. B., S. K. Fyfe & E. M. Wiseman, 1979. Testing F1 hybrids of Brassica oleracea for sibs by seed isoenzyme analysis. Ann. Appl. Biol. 91: 263–270.

    Google Scholar 

  • Wills, A. B. & E. M. Wiseman, 1980. Acid phosphatase isoenzymes of Brassica oleracea seedlings and their applications to sib testing in F1 hybrids. Ann. Appl. Biol. 94: 137–142.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Servei d'Investigació Agrària, Carretera de Cabrils s/n, Cabrils, (Barcelona), Spain

    P. Arús (Generalitat de Catalunya)

  2. Department of Agronomy and Range Science, University of California, 95616, Davis, CA, USA

    C. R. Shields

  3. Applied Genetics Laboratory, Agrigenetics Corporation, 3375 Mitchell Lane, 80301, Boulder, CO, USA

    T. J. Orton

Authors
  1. P. Arús
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. R. Shields
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. J. Orton
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arús, P., Shields, C.R. & Orton, T.J. Application of isozyme electrophoresis for purity testing and cultivar identification of F1 hybrids of Brassica oleracea . Euphytica 34, 651–657 (1985). https://doi.org/10.1007/BF00035400

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00035400

Index words

Search

Navigation