Abstract
A method for rapid and highly effective plant micropropagation from vegetative meristems was established for Aloe barbadensis Mill. Plant micropropagation was achieved culturing apices on medium containing 1.1 μM 2,4-dichlorophenoxyacetic acid and 2.3 μM kinetin for 15–30 days. High morphogenetic ability was maintained by transferring explants (after 60 days) on media containing 0.11 μM 2,4-dichlorophenoxyacetic acid and 2.2 μM 6-benzylaminopurine.
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References
Castorena Sanchez I, Natali L, Cavallini A (1988) In vitro culture of Aloe barbadensis Mill.: morphogenetic ability and nuclear DNA content. Plant Sci 55: 53–59
D'Amato F (1985) Cytogenetics of plant cell and tissue cultures and their regenerates. CRC Crit Rev Plant Sci 3: 73–112
Fassina G (1974) Lezioni di Farmacognosia—Droghe vegetali. CEDAM, Padova, Italy
Groenewald EG, Koeleman A, Wessels DCJ (1975) Callus formation and plant regeneration from seed tissue of Aloe pretoriensis Pole Evans. Z Pflanzenphysiol 75: 270–272
Keijzer CJ, Cresti M (1987) A comparison of anther tissue development in male sterile Aloe vera, and male fertile Aloe ciliaris. Ann Bot 59: 533–542
Larkin PJ, Scowcroft WR (1981) Somaclonal variation: a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15: 473–497
Racchi ML (1987) Plant regeneration from callus cultures of Aloe ferox. Proc Int Congr Plant Tissue Culture, Bogota (in press)
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Natali, L., Sanchez, I.C. & Cavallini, A. In vitro culture of Aloe Barbadensis Mill.: Micropropagation from vegetative meristems. Plant Cell Tiss Organ Cult 20, 71–74 (1990). https://doi.org/10.1007/BF00034761
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DOI: https://doi.org/10.1007/BF00034761