Abstract
Single-node stem segments of Rosmarinus officinalis L. var. genuina forma erectus proved better explants than shoot tips (ca. 2 cm long) for extablishment of field-grown plants in aseptic cultures. Benzylaminopurine was far more effective than kinetin for shoot induction in shoot tips excised from aseptically-grown plants. Maximum numbers of shoot buds (ca. 14) were formed per explant at 0.2 mgl-1 benzylaminopurine in 30 days. After further growth of isolated shoots and treatment with 0.25 mgl-1 indolepropionic acid for 7 days, 80% shoots produced roots. In vitro raised plantlets were successfully grown in soil to plants. About 5,000 plants could be produced from a single nodal segment in 1 year.
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NBRI Research Publication No. 195 (N.S.)
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Misra, P., Chaturvedi, H.C. Micropropagation of Rosmarinus officinalis L.. Plant Cell Tiss Organ Cult 3, 163–168 (1984). https://doi.org/10.1007/BF00033737
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DOI: https://doi.org/10.1007/BF00033737