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Analytical determination of orthophosphate in water

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Abstract

Methods for orthophosphate determination and the problems of interferences are reviewed.

An important group of methods utilizes the phosphomolybdate complex. The complexation step, the reduction step and the extraction step are treated separately and alternative procedures compared.

Another group of methods uses ion association complexes; they are primarily used in physiology and not commonly used in water analyses today.

Enzymatic methods for orthophosphate analysis in natural waters have been developed lately and are ready for application in selected waterbodies.

Flame spectroscopic, fluorometric, gas chromatographic, ion exclusion chromatographic, inductively coupled plasma and other methods are also shortly presented.

Radiobiological bioassays for orthophosphate are also available.

In conclusion it was emphasized that the most common and reliable technique still is the molybdenum blue method as modified by Murphy & Riley (1962).

The need for more specific and sensitive methods is particularly strong in investigations of phosphorus turnover and phosphorus limitation in natural waters. For these purposes the enzymatic phosphatase methods has advantages due to their specificity for orthophosphate and they might offer an alternative to the molybdenum blue method.

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References

  • Ahlgren, G., 1970. Test av analysmetoder för fosfatfosfor. Inst. of Limnology Univ. Uppsala, 17 pp., (in Swedish).

    Google Scholar 

  • Akamatzu, S., 1952. Some micromethods for enzyme studies. J. Biochem. (Tokyo) 39: 203–210.

    Google Scholar 

  • Allen, H. E. & R. B. Hahn, 1969. Determination of phosphate in natural waters by activation analysis of tungstophosphoric acid. Envir. Sci. Technol. 3: 844–848.

    Google Scholar 

  • Amer. Public Health Assn., 1965. Standard methods for examination of water and wastewater including bottom sediments and sludges (12 th ed.), Amer. Public Health Association, NY, 769 pp.

    Google Scholar 

  • Ammon, R. & K. Hinsberg, 1936. Colorimetrishe Phosphor und Arsensäurebestimmung mit Ascorbinsäure. Hoppe-Seyler's Z. 239: 207–216.

    Google Scholar 

  • Atkins, W. R. G., 1923. The phosphate content of fresh and salt water in its relationship to the growth of the algal plankton. J. mar. biol. Ass. UK 13: 119–150.

    Google Scholar 

  • Bedrosian, A. J., R. K. Skogerboe & G. H. Morrison, 1968. Direct emission spectrographic methods for trace elements in biological materials. Analyt. Chem. 40: 854–860.

    Google Scholar 

  • Boltz, D. F. & C. H. Lueck, 1958. Phosphorus. In D. F. Boltz (ed.), Colorimetric determination of nonmetals. Interscience, NY Chemical Analysis, 8: 29–46.

  • Bowen, H. J. M., 1967. Comparative elemental analyses of standard plant material. Analyst 92: 124–131.

    Google Scholar 

  • Burchfield, H. P., D. E. Johnson, J. W. Rhoades & R. J. Wheeler, 1965. Selective detection of phosphorus, sulfur and halogen compounds in the gas chromatograpy of drugs and pesticides. J. Gaschromatogr. 3: 28–34.

    Google Scholar 

  • Burton, J. D. & J. P. Riley, 1956. Determination of soluble phosphate and total phosphorus in sea-water and total phosphorus in marine muds. Mikrochim. Acta: 1350–1365.

  • Burton, J. D., 1973. Problems in the analysis of phosphorus compounds Wat. Res. 7: 291–307.

    Google Scholar 

  • Campbell, F. R. & R. F. Thomas, 1970. Automated method for determining and removing silica interference in determination of soluble phosphorus in lake and stream waters. Envir. Sci. Technol. 4: 602–604.

    Google Scholar 

  • Chamberlain, W. M. & J. Shapiro, 1969. On the biological significance of phosphate analysis; comparison of standard an new methods with a bioassay. Limnol. Oceanogr. 14: 921–927.

    Google Scholar 

  • Chamberlain, W. M. & J. Shapiro, 1973. Phosphate measurements in natural waters — A critique. In E. J. Griffith, A. M. Beeton, J. M. Spencer & D.I. Mitchell (eds.), Environmental Phosphorus Handbook, J. Wiley & Sons, NY: 355–366.

    Google Scholar 

  • Chaube, A. & V. K. Gupta, 1983. Spectrophotometric determination of phosphate in polluted waters by solvent extraction of molybdenum blue. Analyst 108: 1141–1144.

    Google Scholar 

  • Crouch, S. R. & H. V. Malmstadt, 1967. A mechanistic investigation of molybdenum blue method for determination of phosphate Analyt. Chem. 39: 1084–1089.

    Google Scholar 

  • Czensny, R., 1932. Zweckmässige Ausgestaltung der quantitativen Wasseranalyse zur Beurteilung fischereilicher Belange. Die Bestimmungen des pH-Wertes, des Eisens, des Ammoniaks, der Nitrite, der Kiselsäure, der Phosphorsäure und der Sulfate im Wasser. Z. Fisch. 30: 647–689.

    Google Scholar 

  • Czensny, R., 1960. Wasser-, Abwasser- und Fischereichemie. VEB Verlag Tecknik, Berlin, 429 pp.

    Google Scholar 

  • Denigés, G., 1920. Reaction de coloration extremement sensible des phosphates et des arseniates. Ses applications. C.r. Acad. Sci. Paris, 171: 802–804.

    Google Scholar 

  • Dick, W. A. & M. A. Tabatabai, 1977. Determination of orthophosphate in aqueous solutions containing labile organic and inorganic phosphorus compounds. J. envir. Qual. 6: 82–85.

    Google Scholar 

  • Downes, M. T., 1978. Automated determination of low reactive phosphorus concentrations in natural waters in presence of arsenic, silicon and mercuric chloride. Wat. Res. 12: 743–745.

    Google Scholar 

  • Edwards, G. P., A. H. Molof & R. Schneeman, 1965. Determination of orthophosphate in fresh and saline waters. J. am. Wat. Wks. Ass. 57: 917–925.

    Google Scholar 

  • Fiske, C. H. & Y. Subbarow, 1925. The colorimetric determination of phosphorus. J. Biol. Chem. 66: 375–400.

    Google Scholar 

  • Furman, N. H. (ed.), 1962. Scott's standard methods of chemical analysis, 6th ed. vol. I, van Nostrand, Princeton, NJ, 814 pp.

    Google Scholar 

  • Garen, A. & O. Levinthal, 1960. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. Biochim. Biophys. Acta 38: 470–483.

    Google Scholar 

  • Golterman, H. L., 1960. Studies on the cycle of elements in fresh water Acta Bot. Neerlandica 9: 1–58.

    Google Scholar 

  • Golterman, H. L. & I. M. Wurtz, 1961. A sensitive rapid determination of inorganic phosphate in presence of labile phosphate esters Analyt. chim. Acta 25: 295–297.

    Google Scholar 

  • Greenfield, L. J. & F. A. Kalber, 1955. Inorganic phosphate measurement in sea water. Bull. mar. Sci. Gulf Caribb. 4: 323–335.

    Google Scholar 

  • Griffith, E. J., A. M. Beeton, J. M. Spencer & D. I. Mitchell, 1973. (eds.) Environmental phosphorus handbook. J. Wiley & Sons, NY, 718 pp. Guyon, J. G. & W. D. Shults, 1969. Rapid phosphate determination by fluorimetry. J. am. Wat. Wks Ass. 61: 403–404.

    Google Scholar 

  • Hahn, R. B. & T. M. Schmitt, 1969. Determination of phosphate in water with tungsten-185. Analyt. Chem. 41: 359–360.

    Google Scholar 

  • Harwood, J. E. & W. H. J. Hattingh, 1973. Colorimetric methods of analysis of phosphorus at low concentrations in water. In E. J. Griffith, A. M. Beeton, J. M. Spencer & D. I. Mitchell (eds), Environmental Phosphorus Handbook, J. Wiley & Sons, NY: 289–302.

    Google Scholar 

  • Harwood, J. E., R. A. van Steenderen & A. L. Kuhn, 1969. A comparison of some methods for total phosphate analysis. Wat. Res. 3: 425–432.

    Google Scholar 

  • Holzbecher, I. & D. E. Ryan, 1973. The fluorimetric determination of phosphate with thiamine. Analyt. chim. Acta 64: 147–150.

    Google Scholar 

  • Itaya, K. & M. Ui, 1966. A new micromethod for the colorimetric determination of inorganic phosphate. Clin. chim. Acta 14: 361–366.

    Google Scholar 

  • Jackson, M. L., 1958. Soil chemical analysis. Prentice Hall, NY, 498 pp.

    Google Scholar 

  • Janse, T. A. H. M., P. F. A. van Der Wiel & G. Kateman, 1983. Experimental optimization procedures in the determination of phosphate by flow-injection analysis. Analyt. chim. Acta: 89–102.

  • Jean, M., 1955. Sur la formation el la reduction de l'acide phosphomolybdique et de l'acide arsen omolybdique. C.r. Acad. Sci. Paris, 240: 22–37.

    Google Scholar 

  • Jones, P. G. W. & C. P. Spencer, 1963. Comparison of several methods of determining inorganic phosphate in sea water. J. mar. biol. Ass. UK 43: 251–273.

    Google Scholar 

  • Juday, C., E. A. Birge, G. I. Kemmerer & R. J. Robinson, 1928. Phosphorus content of lake waters of northeastern Wisconsin. Trans. Wis. Acad. Sci. 23: 233–248.

    Google Scholar 

  • Kaelble, E. F. (ed.), 1967. Handbook of x-rays, McGraw-Hill, NY, 339 pp.

    Google Scholar 

  • Kaelble, E. F., 1973. Spectrometric methods for determining phosphorus and its compounds. In E. J. Griffith, A. M. Beeton, J. M. Spencer & D. I. Mitchell (eds), Environmental Phosphorus Handbook, J. Wiley & Sons, NY: 341–350.

    Google Scholar 

  • Kallner, A., 1975. Determination of phosphate in serum and urine by a single step Malachite-Green method. Clin. chim. Acta. 59: 35–39.

    Google Scholar 

  • Karmen, A., 1969. Differential specificity in detecting phosphorus, nitrogen, and halogens with alkali flames. J. chromatogr. Sci. 7: 541–549.

    Google Scholar 

  • King, E. J., 1932. The colorimetric determination of phosphorus. Biochem. J. 26: 292–297.

    Google Scholar 

  • Kirkbright, G. F., R. Narayanaswamy & T. S. West, 1971. Spectrofluorimetric determination of orthophosphate as Rodamine B molybdophosphate. Analyt. Chem. 43: 1434–1438.

    Google Scholar 

  • Kirkbright, G. F., R. Narayanaswamy & T. S. West, 1972. Spectrofluorimetric determination of orthophosphate as quininemolybdophophate. Analyst 97: 174–181.

    Google Scholar 

  • Kuenzeler, E. J. & B. H. Ketchum, 1962. Rate of phosphorus uptake by Pheodactylum tricornutum. Biol. Bull. 123: 134–145.

    Google Scholar 

  • Levine, H., J. J. Rowe & F. S. Grimaldi, 1955. Molybdenum blue reaction and determination of phosphorus in waters containing arsenic silicon, and germanium. Analyt. Chem. 27: 258–262.

    Google Scholar 

  • Lei, W., K. Fujiwara & K. Fuwa, 1983. Determination of phosphorus in natural waters by Long-capillary-cell adsorption spectrometry. Analyt. Chem. 55: 951–955.

    Google Scholar 

  • Martin, J. B. & D. M. Doty, 1949. Determination of inorganic phosphate Analyt. Chem. 21: 965–967.

    Google Scholar 

  • Matthews, D. R., W. D. Shults, M. R. Guerin & J. A. Dean, 1971. Extraction — Derivatization - — Gaschromatograpic determination of trace phosphate in aqueous media. Analyt. Chem. 43: 1582–1585.

    Google Scholar 

  • Mc Cune, H. W. & G. J. Arquette, 1955. Precipitation of pyrophosphate and triphosphate with tris (ethylenediamine) cobalt (III)chloride and hexaminecobalt(III)-chloride. Analyt. Chem. 27;; 401–405.

    Google Scholar 

  • Motomizu, S., T. Wakimoto & K. Toei, 1984. a. Solvent extraction — spectrophotometric determination of phosphate with molybdate and Malachite Green in river and sea-water. Talanta, 31: 235–240.

    Google Scholar 

  • Motomizu, S., H. Mikasa, M. Oshima & K. Toei, 1984. b. Continuous flow method for the determination of phosphorus using the fluorescence quenching or Rhodamine 6 G with molybdophosphate. Buneski Kagaku 33: 116–118.

    Google Scholar 

  • Murphy, J. & J. P. Riley, 1958. A single-solution method for the determination of soluble phosphate in sea-water. J. mar. biol. Ass. UK 37: 235–240.

    Google Scholar 

  • Murphy, J. & J. P. Riley, 1962. A modified single-solution method for the determination of phosphate in natural waters. Analyt. chim. Acta 27: 31–36.

    Google Scholar 

  • Namiki, H., 1961. Spectrophotometric determination of phosphate in sewage by extraction of molybdenum blue. Bunseki Kagaku 10: 945–951.

    Google Scholar 

  • Ohle, W., 1938. Zur Vervollkommnung der hydrochemishen Analyse III. Die Phosphorbestimmung. Z. angew. Chem. 51: 906–911.

    Google Scholar 

  • Olsen, S., 1967. Recent trends in the determination of orthophosphate in water. In H. L. Golterman & R. S. N. V. Clymo (eds), Chemical Environment in the Aquatic Habitat. Noord-Hollandsche uitgevers Maatschappij, Amsterdam: 63–105.

    Google Scholar 

  • Osmond, F., 1987. Sur une reaction pouvant sevir en dosage colorimetrique du phosphore dans les fontes, les aciers. Bull. Soc. chim., Paris. 47: 745–748.

    Google Scholar 

  • Pettersson, K., 1979. Enzymatic determination of orthophosphate in natural waters. Int. Revue ges. Hydrobiol. 64: 585–607.

    Google Scholar 

  • Pierce, W. C., E. L. Hoenish & D. T. Sawyer, 1959. Quantitative analysis, 4th. ed., J. Wiley & Sons. NY: 369–373.

    Google Scholar 

  • Proctor, C. M. & D. W. Hood, 1954. Determination of inorganic phosphate in sea water by an iso-butanol extraction procedure. J. mar. Res. 13: 122–132.

    Google Scholar 

  • Rigler, F. H., 1966. Radiobiological analysis of inorganic phosphorus in lake water. Verh. int. Ver. Limnol. 16: 465–470.

    Google Scholar 

  • Shapiro, J., 1971. Arsenic and phosphate: Measured by various techniques. Science, 171: 234.

    Google Scholar 

  • SIS, 1984. Vattenundersökningar bestämmning av fosfat (SIS.028126). SIS, Stockholm, 8 pp. (in Swedish).

    Google Scholar 

  • Stainton, M. P., 1980. Errors in the molybdenum blue methods for determining orthophosphate in fresh water. Can. J. Fish aquat. Sci. 37: 472–478.

    Google Scholar 

  • Stephens, K., 1963. Determination of low phosphate concentrations in lake and marine waters. Limnol. Oceanogr. 8: 361–362.

    Google Scholar 

  • Stevens, R. J., 1979. Evaluation of an enzymatic method for orthophosphate determination in freshwaters. Wat. Res. 13: 763–770.

    Google Scholar 

  • Strickland, J. D. H. & T. R. Parsons, 1965. A manual of sea water analysis. Bull. Fish Res. Bd Can. No. 125. 185 pp.

  • Strickland, J. D. H. & T. R. Parsons, 1968. A practical handbook of seawater analysis. Bull. Fish Res. Bd Can. No. 167. 185 pp.

  • Strickland, J. D. H. & L. Solorzano, 1966. Determination of monoesterase hydrolysable phosphate and phosphomonoesterase activity in sea water. In H. Barnes (ed.), Some contemporary studies in Marine Science. Allen & Unwin, Lond. 665–675.

    Google Scholar 

  • Sugawara, K. & S. Kanamori, 1961. Spectrophotometric determination of submicrogram quantities of orthophosphate in natural waters. Bull. chem. Soc. Japan, 34: 258–261.

    Google Scholar 

  • Taft, J. L., M. E. Loftus & W. R. Taylor, 1977. Phosphate uptake from phosphomonoesters by phytoplankton in the Chesapeake Bay. Limnol. Oceanogr. 22: 1013–1021.

    Google Scholar 

  • Tanaka, K. & T. Ishizuka, 1982. Determination of phosphate in wastewaters by Ion-exclusion-chromatography with flow colometric detection. Wat. Res. 16: 719–723.

    Google Scholar 

  • Tyree, Jr., S. Y. & M. A. O. Bynum, 1984. A method for nitrate and phosphate in saline water. Limnol. Oceanogr. 29: 1337–1339.

    Google Scholar 

  • Wagner, W., C. J. Hull & G. E. Markle, 1956. Advanced Analytical Chemistry, Reinhold, NY, 282 pp.

    Google Scholar 

  • Wayman, C. H., 1964. Simultaneous determination of sulphur and phosphorus in water by neutron activation analysis. Analyt. Chem. 36: 665–666.

    Google Scholar 

  • van Wazer, J. R., 1958. Phosphorus and its compounds. I Chemistry., II: Technology, biological functions, and applications. Interscience, NY, 2046 pp.

    Google Scholar 

  • Whatly, T. P. & L. W. Ferrara, 1973. Gravimetric Analysis of phosphorus compounds. In E. J. Griffith, A. M. Beeton, J. M. Spencer & D. I. Mitchell (eds.), Environmental Phosphorus Handbook, J. Wiley & Sons, NY: 313–326.

    Google Scholar 

  • Wilson, H. N., 1954. The determination of phosphate in the presence of soluble silicates. Analyst 79: 535–546.

    Google Scholar 

  • Vogler, P., 1965. Beiträge zur Phosphatanalytik in der Limnologie II. Die Bestimmung des gelösten Orthophosphates. Fortschr. Wasserchem. 109–119.

  • Won, C. H., 1964. Sensitive spectrophotometric determination of orthophosphate in natural waters by a modified molybdenum (V) thiocyanate method. Nippon Kagaku Zasshi 85: 859–863.

    Google Scholar 

  • Zaugg, W. S., 1967. Determination of phosphate in fresh and seawater by atomic absorption spectrophotometry Atomic Absorpt. Newslett. 6: 63–65.

    Google Scholar 

  • Zaugg, W. S. & R. J. Knox, 1966. Indirect determination of iorganic phosphate by atomic absorption spectrophotometric determination of molybdenum. Analyt. Chem. 38: 1759–1760.

    Google Scholar 

  • Zimmerman, P., 1961. Chemische und bakteriologische Untersuchung im unteren Zurichsee während der Jahre 1948–1957. Schweiz. Z. Hydrol. 23: 342–397.

    Google Scholar 

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  1. Department of Limnology, Uppsala University, Box 557, S-751 22, Uppsala, Sweden

    Ola Broberg & Kurt Pettersson

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Broberg, O., Pettersson, K. Analytical determination of orthophosphate in water. Hydrobiologia 170, 45–59 (1988). https://doi.org/10.1007/BF00024898

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