Summary
The potential of isozymes for distinguishing asparagus varieties was carried out by a survey on 21 varieties using 10 enzyme systems: GOT, SkDH, DIA, PGM, MDH, IDH, PGD, ACP, PGI, MR and ADH. Only 3 enzymes, SkDH, GOT and PGM, showed useful polymorphisms. The varieties were found heterogeneous according to their genetic structure: open pollinated varieties were more heterogeneous than clonal hybrids; the F1 hybrid and the vitroclones were homogeneous. As expected from the narrow genetic basis of the varieties, only a few alleles per isozyme locus were present. Moreover, for each enzyme, one allele or type was predominant so that the discriminating power of the method was low. However some of the varieties could be identified and different applications of the results are presented.
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Abbreviations
- D.U.S.-:
-
Distinction-Uniformity-Stability
- ACO-:
-
aconitase
- ACP-:
-
acid phosphatase
- ADH-:
-
alcohol dehydrogenase
- CAT-:
-
catalase
- DIA-:
-
diaphorase
- END-:
-
endopeptidase
- GOT-:
-
glutamate oxaloacetate transaminase
- IDH-:
-
isocitrate dehydrogenase
- MDH-:
-
malate dehydrogenase
- MR-:
-
menadione reductase
- PGI-:
-
phosphoglucoisomerase
- PGM-:
-
phosphoglucomutase
- PGD-:
-
phosphoglucose dehydrogenase
- POX-:
-
peroxidase
- SkDH-:
-
shikimate dehydrogenase
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Lallemand, J., Briand, F., Breuils, F. et al. Identification of asparagus varieties by isozyme patterns. Euphytica 79, 1–4 (1994). https://doi.org/10.1007/BF00023569
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DOI: https://doi.org/10.1007/BF00023569