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cDNA cloning, characterization and expression of an endosperm-specific barley peroxidase

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Abstract

A barley peroxidase (BP 1) of pI ca. 8.5 and M r 37000 has been purified from mature barley grains. Using antibodies towards peroxidase BP 1, a cDNA clone (pcR7) was isolated from a cDNA expression library. The nucleotide sequence of pcR7 gave a derived amino acid sequence identical to the 158 C-terminal amino acid residues of mature BP 1. The clone pcR7 encodes an additional C-terminal sequence of 22 residues, which apparently are removed during processing. BP 1 is less than 50% identical to other sequenced plant peroxidases. Analyses of RNA and protein from aleurone, endosperm and embryo tissue showed maximal expression 15 days after flowering, and high levels were found only in the endosperm. BP 1 was not expressed in the leaves.

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Authors and Affiliations

  1. Plant Biology Section, Risø National Laboratory, DK-4000, Roskilde, Denmark

    Søren K. Rasmussen

  2. Institute of Biochemical Genetics, University of Copenhagen, 2A Ø. Farimagsgade, DK-1353, Copenhagen, Denmark

    Karen G. Welinder

  3. Department of Biochemistry and Nutrition, Technical University of Denmark, DK-2800, Lyngby, Denmark

    Jørn Hejgaard

Authors
  1. Søren K. Rasmussen
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  2. Karen G. Welinder
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  3. Jørn Hejgaard
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Rasmussen, S.K., Welinder, K.G. & Hejgaard, J. cDNA cloning, characterization and expression of an endosperm-specific barley peroxidase. Plant Mol Biol 16, 317–327 (1991). https://doi.org/10.1007/BF00020562

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  • DOI: https://doi.org/10.1007/BF00020562

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