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Cloning of a pea cDNA encoding a polypeptide of the light-harvesting complex associated with photosystem I using a monoclonal antibody

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Abstract

A monoclonal antibody (MAb UB42) is described that binds to thylakoids in pea chloroplasts, as shown by EM-immunogold labelling. The antibody recognised proteins of ca. 23–29 kDa in western blots of a pea leaf homogenate. A cDNA library was prepared from pea epidermal cells in the vector λZAP II, and immunoscreening of the library with UB42 led to the isolation of a clone, pUB42. This was sequenced and had an open reading frame of 269 codons encoding a predicted polypeptide of 28.9 kDa. The sequence showed extensive homology with three closely related polypeptides belonging to a family of chlorophyll a/b-binding proteins from the light harvesting complex of photosytem I (LHCI). Collectively, the results suggest that MAb UB42 recognises an epitope on the type II chlorophyll a/b-binding protein from LHCI and that clone pUB42 encodes this protein.

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Authors and Affiliations

  1. School of Biological Sciences, University of Birmingham, PO Box 363, B15 2TT, Birmingham, UK

    Martin E. Cannell, Alison J. Mitchell, Sharon McCready, James A. Callow & Jonathan R. Green

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  1. Martin E. Cannell
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  2. Alison J. Mitchell
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  3. Sharon McCready
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  4. James A. Callow
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  5. Jonathan R. Green
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Cannell, M.E., Mitchell, A.J., McCready, S. et al. Cloning of a pea cDNA encoding a polypeptide of the light-harvesting complex associated with photosystem I using a monoclonal antibody. Plant Mol Biol 27, 821–824 (1995). https://doi.org/10.1007/BF00020235

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  • DOI: https://doi.org/10.1007/BF00020235

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