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Identification of legumin-like proteins in wheat

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Abstract

We have obtained several amino acid sequences from purified polypeptides of a wheat endosperm storage globulin previously described as ‘triplet’ protein. The amino acid sequence data supported by immunochemical analysis using anti-oat 12S globulin antibodies, provide definitive evidence that the triplet protein is homologous to pea legumin and related seed storage proteins of oats, rice and several dicotyledonous species. Thus, it is now proposed that the triplet protein of wheat be renamed ‘triticin’. The oat globulin antibodies also cross-reacted strongly with the high-molecular-weight (HMW) glutenin subunits which have been implicated in bread-making quality.

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Authors and Affiliations

  1. Agronomy Department, Waite Agricultural Research Institute, 5064, Glen Osmond, South Australia, Australia

    Nagendra K. Singh & Kenneth W. Shepherd

  2. Agricultural Biochemistry Department, Waite Agricultural Research Institute, 5064, Glen Osmond, South Australia, Australia

    Nagendra K. Singh & Peter Langridge

  3. CSIRO Division of Biotechnology, 3052, Parkville, Victoria, Australia

    Nagendra K. Singh & L. Clem Gruen

  4. (Wheat Research Unit), CSIRO Division of Plant Industry, 2113, North Ryde, N.S.W., Australia

    John H. Skerritt & Colin W. Wrigley

Authors
  1. Nagendra K. Singh
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  2. Kenneth W. Shepherd
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  3. Peter Langridge
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  4. L. Clem Gruen
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  5. John H. Skerritt
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  6. Colin W. Wrigley
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Singh, N.K., Shepherd, K.W., Langridge, P. et al. Identification of legumin-like proteins in wheat. Plant Mol Biol 11, 633–639 (1988). https://doi.org/10.1007/BF00017463

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  • DOI: https://doi.org/10.1007/BF00017463

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