Abstract
The present chapter discusses the various dye for the staining of nucleic acid in flow cytometry. The DNA-binding dye should be specific for DNA and should bind stoichiometric to the DNA content. With the help of DNA flow cytometry one can assess the ploidy and cell proliferative fraction of tumour.
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References
Shankey TV, Rabinovitch PS, Bagwell B, Bauer KD, Duque RE, Hedley DW, Mayall BH, Wheeless L, Cox C. Guidelines for implementation of clinical DNA cytometry. International Society for Analytical Cytology Cytometry. 1993;14(5):472–7.
Ormerod MG, Tribukait B, Giaretti W. Consensus report of the task force on standardisation of DNA flow cytometry in clinical pathology. DNA flow cytometry task force of the European Society for Analytical Cellular Pathology. Anal Cell Pathol. 1998;17(2):103–10.
Vindeløv LL, Christensen IJ, Nissen NI. A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry. 1983 Mar;3(5):323–7.
Darzynkiewicz Z, Huang X, Zhao H. Analysis of cellular DNA content by flow cytometry. Curr Protoc Immunol. 2017 Nov 1;119:5.7.1–5.7.20.
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Dey, P. (2021). Nuclei Acid Dye and DNA Content Measurement in Flow Cytometry. In: Diagnostic Flow Cytometry in Cytology. Springer, Singapore. https://doi.org/10.1007/978-981-16-2655-5_7
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DOI: https://doi.org/10.1007/978-981-16-2655-5_7
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