Skip to main content
SpringerLink
Log in

Characteristics and Empirical Modelling of Extract from Hibiscus sabdariffa Using Supercritical CO2 Extraction with Ethanol-Water as Modifier

  • Conference paper
  • First Online:
Proceedings of the 3rd International Conference on Separation Technology

Part of the book series: Lecture Notes in Mechanical Engineering ((LNME))

  • 359 Accesses

Abstract

The main constituents of the extract from Hibiscus sabdariffa calyces are anthocyanins which gave intense natural red colorant and commonly used for centuries for therapeutic purposes. Supercritical carbon dioxide (SC-CO2) technique could produce high-quality extracts from plant materials as it can exploit the non-toxic character and the gas–liquid properties of the SC-CO2. This research aimed to characterise the colour and the pattern of Hibiscus sabdariffa SC-CO2 extraction. The colour was evaluated by using a CIELAB method meanwhile the presence of anthocyanins in the red colour extract was confirmed by High-Performance Liquid Chromatograph (HPLC). Two types of empirical models (Modified Brunner’s and Esquivel’s models) were used to describe the extracts obtained at series of operational parameters (i.e. pressure and temperature) at 7.5 and 10% of modifier ratio, with a total solvent flow rate of 4 ml/min. Both models were analysed using a non-linear regression method. The modified Brunner’s model shows the best agreement between experimental and model calculated data with the lowest AARD of 1.30% at 70 °C, 10 MPa, and 10% modifier ratio.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Riaz G, Chopra R (2018) A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L. Biomed Pharmacother 102:575–586

    Article  Google Scholar 

  2. Kao ES, Yang MY, Hung CH, Huang CN, Wang CJ (2016) Polyphenolic extract from Hibiscus sabdariffa reduces body fat by inhibiting hepatic lipogenesis and preadipocyte adipogenesis. Food Funct 7(1):171–182

    Article  Google Scholar 

  3. Andzi Barhé T, Feuya Tchouya GR (2016) Comparative study of the anti-oxidant activity of the total polyphenols extracted from Hibiscus Sabdariffa L., Glycine max L. Merr., yellow tea and red wine through reaction with DPPH free radicals. Arab J Chem 9(1):1–8

    Google Scholar 

  4. Da-Costa-Rocha I, Bonnlaender B, Sievers H, Pischel I, Heinrich M (2014) Hibiscus sabdariffa L.—a phytochemical and pharmacological review. Food Chem 165:424–443

    Google Scholar 

  5. Losada-Barreiro S, Bravo-Díaz C (2017) Free radicals and polyphenols: the redox chemistry of neurodegenerative diseases. Eur J Med Chem 133:379–402

    Article  Google Scholar 

  6. Chatterjee D, Jadhav NT, Bhattacharjee P (2013) Solvent and supercritical carbon dioxide extraction of color from eggplants: characterization and food applications. LWT—Food Sci Technol 51(1):319–324

    Article  Google Scholar 

  7. del Pilar Garcia-Mendoza M, Espinosa-Pardo FA, Baseggio AM, Barbero GF, Maróstica Junior MR, Rostagno MA, Martinez J (2017) Extraction of phenolic compounds and anthocyanins from juçara (Euterpe edulis Mart.) residues using pressurized liquids and supercritical fluids. J Supercrit Fluids 119:9–16

    Google Scholar 

  8. Nobre BP, Mendes RL, Queiroz EM, Pessoa FLP, Coelho JP, Palavra AF (2006) Supercritical carbon dioxide extraction of pigments from bixa orellana seeds (experiments and modeling). Braz J Chem Eng 23(2):251–258

    Article  Google Scholar 

  9. Talib N, Ahmad MR, Ismail K, Ab Kadir MI (2016) Comparison of supercritical fluid extraction and ultrasound-assisted extraction of natural dyes from a brown seaweed (Sargassum spinosum). Int J Text Sci 5(6):141–144

    Google Scholar 

  10. Gadkari PV, Balaraman M (2015) Extraction of catechins from decaffeinated green tea for development of nanoemulsion using palm oil and sunflower oil based lipid carrier systems. J Food Eng 147:14–23

    Article  Google Scholar 

  11. Kumar S (2015) Analytical techniques in natural product research. In: Analytical techniques for natural product research. CABI, Wallingford, United Kingdom, pp 1–10

    Google Scholar 

  12. Lukmanto S, Roesdiyono N, Ju YH, Indraswati N, Soetaredjo FE, Ismadji S (2013) Supercritical CO2 extraction of phenolic compounds in Roselle (Hibiscus Sabdariffa L.). Chem Eng Commun 200(9):1187–1196

    Google Scholar 

  13. Pimentel-Moral S, Borrás-Linares I, Lozano-Sánchez J, Arráez-Román D, Martínez-Férez A, Segura-Carretero A (2019) Supercritical CO2 extraction of bioactive compounds from Hibiscus sabdariffa. J Supercrit Fluids 147:213–221

    Article  Google Scholar 

  14. Idham Z, Nasir HMM, Yunus MAC, Yian LN, Peng WL, Hassan H, Mohd Setapar SH (2017) Optimisation of supercritical CO2 extraction of red colour from roselle (Hibiscus Sabdariffa Linn.) calyces. Chem Eng Trans 56:871–876

    Google Scholar 

  15. Comim SRR, Madella K, Oliveira JV, Ferreira SRS (2010) Supercritical fluid extraction from dried banana peel (Musa spp., genomic group AAB): extraction yield, mathematical modeling, economical analysis and phase equilibria. J Supercrit Fluids 54(1):30–37

    Google Scholar 

  16. Zeković Z, Filip S, Vidović S, Jokić S, Svilović S (2014) Mathematical modeling of ocimum basilicum L. supercritical CO2 extraction. Chem Eng Technol 37(12):2123–2128

    Google Scholar 

  17. Putra NR, Idham Z, Machmudah S, Ruslan MSH, Che Yunus MA (2018) Extraction of peanut skin oil by modified supercritical carbon dioxide: empirical modelling and optimization. Sep Sci Technol 53(17):2695–2703

    Google Scholar 

  18. Brunner G (1984) Mass transfer from solid material in gas extraction. Ber der Bunsenges/Phys Chem Chem Phys 88(9):887–891

    Google Scholar 

  19. Esquível MM, Bernardo-Gil MG, King MB (1999) Mathematical models for supercritical extraction of olive husk oil. J Supercrit Fluids 16:43–58

    Article  Google Scholar 

  20. Brunner G (1996) Gas extraction—an introduction to fundamentals of supercritical fluids and the application to separation processes. Topics in physical chemistry. In: Baumgärtel H, Franck EU, Grunbein W (eds) Berichte der Bunsengesellschaft für physikalische Chemie, vol 4. Springer, New York LLC

    Google Scholar 

  21. Jokic S, Svilovic S, Vidovic S (2015) Modelling the supercritical CO2 extraction kinetics of soybean oil. Croat J Food Sci Technol 7(2):52–57

    Article  Google Scholar 

  22. Maran JP, Priya B, Manikandan S (2014) Modeling and optimization of supercritical fluid extraction of anthocyanin and phenolic compounds from Syzygium cumini fruit pulp. J Food Sci Technol 51(9):1938–1946

    Article  Google Scholar 

  23. Fragoso-Jiménez J, Tapia-Campos E, Estarron-Espinosa M, Barba-Gonzalez R, Castañeda-Saucedo M, Castillo-Herrera G (2019) Effect of supercritical fluid extraction process on chemical composition of Polianthes tuberosa flower extracts. Processes 7(2):60

    Article  Google Scholar 

  24. Hasmida MN, Liza MS, Nur Syukriah AR, Harisun Y, Mohd Azizi CY, Fadzilah Adibah AM (2015) Total phenolic content and antioxidant activity of Quercus infectoria galls using supercritical CO2 extraction technique and its comparison with Soxhlet extraction. Pertanika J Sci Technol 23(2):287–295

    Google Scholar 

  25. Ruslan MSH, Idham Z, Nian Yian L, Ahmad Zaini MA, Che Yunus MA (2018) Effect of operating conditions on catechin extraction from betel nuts using supercritical CO2-methanol extraction. Sep Sci Technol 53(4):662–670

    Google Scholar 

  26. Vallecilla-Yepez L, Ciftci ON (2018) Increasing cis-lycopene content of the oleoresin from tomato processing byproducts using supercritical carbon dioxide. LWT—Food Sci Technol 95:354–360

    Article  Google Scholar 

Download references

Acknowledgements

All authors would like to acknowledge the Fundamental Research Grant Scheme, vote number: 4F997 (FRGS/1/2018/STG07/UTM/02/13) from Minister of Higher Education and Universiti Teknologi Malaysia for the UTMLead scholarship and UTM Industry International Incentive Grant (01M76) for financial support. We were also grateful to the Centre of Lipids Engineering and Applied Research (CLEAR), UTM for the facilities provided.

Author information

Authors and Affiliations

  1. Centre of Lipids Engineering and Applied Research, Ibnu Sina Institute for Scientific & Industrial Research, Universiti Teknologi Malaysia, Johor Bahru, Malaysia

    Zuhaili Idham, Nicky Rahmana Putra, Nor Faadila Mohd Idrus, Norlisa Mili, Noor Azwani Mohd Rasidek & Mohd Azizi Che Yunus

  2. Faculty of Engineering, School of Chemical and Energy Engineering, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia

    Zuhaili Idham, Nor Faadila Mohd Idrus & Mohd Azizi Che Yunus

  3. Faculty of Engineering, Universiti Malaysia Sarawak (UNIMAS), 94300, Kota Samarahan, Sarawak, Malaysia

    Norlisa Mili

  4. Department of Chemical Engineering, Universiti Teknologi PETRONAS, Bandar Sri Iskandar, Perak, Malaysia

    Muhammad Syafiq Hazwan Ruslan

Authors
  1. Zuhaili Idham
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nicky Rahmana Putra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nor Faadila Mohd Idrus
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Norlisa Mili
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Muhammad Syafiq Hazwan Ruslan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Noor Azwani Mohd Rasidek
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Mohd Azizi Che Yunus
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mohd Azizi Che Yunus .

Editor information

Editors and Affiliations

  1. University of Technology Malaysia, Bahru, Malaysia

    Muhammad Abbas Ahmad Zaini

  2. University of Technology Malaysia, Bahru, Malaysia

    Mazura Jusoh

  3. University of Technology Malaysia, Bahru, Malaysia

    Norasikin Othman

Rights and permissions

Reprints and permissions

Copyright information

© 2021 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

About this paper

Check for updates. Verify currency and authenticity via CrossMark

Cite this paper

Idham, Z. et al. (2021). Characteristics and Empirical Modelling of Extract from Hibiscus sabdariffa Using Supercritical CO2 Extraction with Ethanol-Water as Modifier. In: Zaini, M.A.A., Jusoh, M., Othman, N. (eds) Proceedings of the 3rd International Conference on Separation Technology. Lecture Notes in Mechanical Engineering. Springer, Singapore. https://doi.org/10.1007/978-981-16-0742-4_26

Download citation

  • DOI: https://doi.org/10.1007/978-981-16-0742-4_26

  • Published:

  • Publisher Name: Springer, Singapore

  • Print ISBN: 978-981-16-0741-7

  • Online ISBN: 978-981-16-0742-4

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics

Search

Navigation