Abstract
The calcium phosphate coating was provided onto the titanium substrate because of the nanoarc coatings technology. Both surface morphology and electrical charge of the coating were measured at the nano/micro-scaled lateral resolution. The negative electrical potential was typical for sockets, however the positive one to the peaks of the roughness. The cells were mainly attached at the negatively charged sockets. The cells expressed both osteocalcin and alkaline phosphatase that are the osteoblastic molecular markers.
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Acknowledgements
The authors wish to thank K.V. Zaitsev (Stem Cell Bank Ltd., Tomsk, Russia) for the cell line provision and Rafael Manory for assistance in preparing this article for submission. The work has been partially financially supported by the Russian Science Foundation, Project No. 16-15-10031 (in part of cell culturing in vitro).
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Dekhtyar, Y. et al. (2019). The Biomaterial Surface Nanoscaled Electrical Potential Promotes Osteogenesis of the Stromal Cell. In: Lhotska, L., Sukupova, L., Lacković, I., Ibbott, G. (eds) World Congress on Medical Physics and Biomedical Engineering 2018. IFMBE Proceedings, vol 68/3. Springer, Singapore. https://doi.org/10.1007/978-981-10-9023-3_25
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