Abstract
Untreated industrial effluents discharged into the environment pose a serious problem to the soil living organism and human beings also. Almost all the traditional physicochemical methods do not provide effective solutions for the elimination of metals from industrial effluents. Industrial waste soil samples collected from different industrial belts of West Bengal were analyzed for physicochemical and microbiological characteristics. Eighteen arsenic (As)-tolerant bacterial strains were isolated from arsenic-contaminated industrial soil. Among them, three bacterial strains, viz., MsfL2, HsR21, and HlR211, exhibited higher As resistance capacity and showed 50% relative growth in LB medium incorporated with 1500 μg/ml of arsenate (V) and 200 μg/ml of arsenite (III). Antibiotic susceptibility of the isolates was also done, and results indicate variation with respect to the tested strain. Additionally it was found that the strains were sensitive to gentamycin, tetracycline, and bacitracin. Biochemical analysis and 16S rRNA sequencing were done to identify and determine the phylogeny of the selected arsenic-tolerant strains. Since arsenic-induced stress has often been known to have correlation with osmotic as well as oxidative stress, the selected strains under study might be metabolically adapted to arsenic-induced oxidative and osmotic stress. Physiological test and PCR results indicate that the isolates probably have developed resistance via arsenic reduction mechanism. From all the above facts, it is evident that the selected isolate(s) is promising candidate(s) for arsenic bioremediation in polluted industrial environment.
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Acknowledgment
The authors would like to express their appreciations to Department of Biotechnology, Government of West Bengal, and Department of Microbiology, Barrackpore Rastraguru Surendranath College, for the financial support and research facilities for this work, respectively. We are also thankful to Sri Prabir Barui, Scientist, Pollution Control Board, Government of West Bengal, for his guidance in sampling as well as AAS facility.
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Dutta, P., Mallick, I., Ghosh, A., Basu, M. (2018). Study of Some Predominant Arsenic Resistance Bacteria from Soil Samples of Industrial Zones of West Bengal, India. In: Ghosh, S. (eds) Utilization and Management of Bioresources. Springer, Singapore. https://doi.org/10.1007/978-981-10-5349-8_19
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DOI: https://doi.org/10.1007/978-981-10-5349-8_19
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