Abstract
Gamma ray irradiation causes immune suppressive responses by inducing oxidative stress such as reduction of cell viability and damages in immune cells. In this present study, we investigated whether Octopus ocellatus meet (OM) consisted of a plentiful taurine has protective effects against damages caused by oxidative stress in murine splenocytes. First of all, we prepared the aqueous extract from OM (OMA) and identified it contained a plentiful taurine content. The result also showed that OMA exhibited the antioxidant activity by scavenging DPPH and ABTS+ radicals and hydrogen peroxide. In addition, OMA improved the cell viability without cytotoxicity in gamma ray-irradiated murine splenocytes. Moreover, OMA significantly reduced the production of reactive oxygen species (ROS) in gamma ray-irradiated splenocytes. In further study, we identified that OMA protected zebrafish embryo via improving the reduced survival rate and decreasing the formation of deformity caused by the exposure of gamma ray irradiation. Also, OMA decreased the production of NO and ROS in gamma ray-irradiated zebrafish embryos as well as the induction of cell death. In these results, this study suggests that the consumption of taurine-rich foods, such as O. ocellatus, may be useful for the useful material for the protection against oxidative stress.
$W. Lee and E.A. Kim equally contributed to this study.
§Y.-J. Jeon and G. Ahn equally contributed to this study.
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Abbreviations
- OM:
-
Octopus ocellatus meat
- ROS:
-
Reactive oxygen species
References
Bing SJ, Kim MJ, Park E, Ahn G, Kim DS, Ko RK, Lee NH, Shin T, Park JW, Jee Y (2010) Modulation of radiation-induced apoptosis by 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG) in murine splenocytes. Biol Pharm Bull 33(7):1122–1127
Bladen CL, Lam WK, Dynan WS, Kozlowski DJ (2005) DNA damage response and Ku80 function in the vertebrate embryo. Nucleic Acids Res 33(9):3002–3010
Blois MS (1958) Antioxidant Determinations by the Use of a Stable Free Radical. Nature 181:1199–1200
Boletzky S (1977) Post-hatching behaviour and mode of life in cephalopods. Symp Zool Soc Lond 38:557–567
Byon YY, Kim MH, Yoo ES, Hwang KK, Jee Y, Shin T, Joo HG (2008) Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity. J Vet Sci 9(4):359–365
Checker R, Chatterjee S, Sharma D, Gupta S, Variyar P, Sharma A, Poduval TB (2008) Immunomodulatory and radioprotective effects of lignans derived from fresh nutmeg mace (Myristica fragrans) in mammalian splenocytes. Int Immunopharmacol 8(5):661–669
Curran WJ (1998) Radiation-induced toxicities: the role of radioprotectants. Semin Radiat Oncol 8(4 Suppl 1):2–4
Daroczi B, Kari G, McAleer MF, Wolf JC, Rodeck U, Dicker AP (2006) In vivo radioprotection by the fullerene nanoparticle DF-1 as assessed in a zebrafish model. Clin Cancer Res 12(23):7086–7091
Dimri M, Joshi J, Chakrabarti R, Sehgal N, Sureshbabu A, Kumar IP (2015) Todralazine protects zebrafish from lethal effects of ionizing radiation: role of hematopoietic cell expansion. Zebrafish 12(1):33–47
Geiger GA, Parker SE, Beothy AP, Tucker JA, Mullins MC, Kao GD (2006) Zebrafish as a “biosensor”? Effects of ionizing radiation and amifostine on embryonic viability and development. Cancer Res 66(16):8172–8181
Halliwell B, Aroma OI (1991) DNA damage by oxygen-derived species. Its mechanism and measurement in mammalian systems. FEBS Lett 281(1–2):9–19
Huxtable RJ (1992) Others: physiological actions of taurine. Physiol Rev 72(1):101–163
Hwang M, Yong C, Moretti L, Lu B (2007) Zebrafish as a model system to screen radiation modifiers. Curr Genomics 8(6):360–369
Islambulchilar M, Asvadi I, Sanaat Z, Esfahani A, Sattari M (2015) Effect of taurine on febrile episodes in acute lymphoblastic leukemia. Adv Pharm Bull 5(1):103–108
Kang KA, Zhang R, Lee KH, Chae SW, Kin BJ, Kwak YS, Park JW, Lee NH, Hyun JW (2006) Protective effect of Triphoroethol-A from Ecklonia cava gainst ionidimg radiation. J Radiat Res 47:61–68
Kim HK, Cheon BS, Kim YH, Kim SY, Kim HP (1999) Effects of naturally occurring flavonoids on nitric oxide production in the macrophage cell line RAW 264.7 and their structure-activity relationships. Biochem Pharmacol 58:759–765
Kumar S, Sharma A, Madan B, Singhal V, Ghosh B (2007) Isoliquiritigenin inhibits IkappaB kinase activity and ROS generation to block TNF-alpha induced expression of cell adhesion molecules on human endothelial cells. Biochem Pharmacol 73:1602–1612
Lee W, kang N, Kim EA, Yang HW, Oh JY, Fernando IPS, Kim KN, Kim KN, Ahn G (2016) Radioprotective effects of a polysaccharide purified from Lactobacillus plantarum-fermented Ishige okamurae against oxidative stress caused by gamma ray-irradiation in zebrafish in vivo model. J Functional Foods 28:83–89
Lee WW, Ahn G, Lee BJ, Wijesinghe WA, Kim D, Yang H, Kim YH, Park SJ, Jee Y, Jeon YJ (2013) Radio-protective effect of polysaccharides isolated from Lactobacillus brevis-fermented Ecklonia cava. Int J Biol Macromol 52:260–266
Leong LP, Shui G (2002) An investigation of antioxidant capacity of fruits in singapore markets. Food Chem 76(1):69–75
Marcinkiewicz J, Kurnyta M, Biedroń R, Bobek M, Kontny E, Maśliński W (2006) Antiinflammatory effects of taurine derivatives (taurine chloramine, taurine brGal-Taumine, and taurolidine) are mediated by different mechanisms. Adv Exp Med Biol 583:481–492
McAleer MF, Davidson C, Davidson WR, Yentzer B, Farber SA, Rodeck U, Dicker AP (2005) Novel use of zebrafish as a vertebrate model to screen radiation protectors and sensitizers. Int J Radiat Oncol Biol Phys 61(1):10–13
Miao J, Fa Y, Gu B, Zhu W, Zou S (2012) Taurine attenuates lipopolysaccharide-induced disfunction in mouse mammary epithelial cells. Cytokine 59:35–40
Mueller S, Arnhold J (1995) Fast and densitive chemiluminescence determination of H2O2 concentration in stimulated human neutropjils. Luminescence 10(4):229–237
Niittynen L, Nurminen ML, Korpela R, Vapaatalo H (1999) Role of arginine, taurine and homocysteine in cardiovascular diseases. Annals of Medicine 31:318–326
Okezie IA (1998) Free radicals, oxidative stress and anti-oxidants in human health and disease. JAOES 75:199–212
Ozben T (2007) Oxidative stress and apoptosis: impact on cancer therapy. Pharm J Sci 96:2181–2196
Park E, Ahn G, Lee NH, Kim JM, Yun JS, Hyun JW, Jeon YJ, Wie MB, Lee YJ, Park JW, Jee Y (2008a) Radioprotective properties of eckol against ionizing radiation in mice. FEBS Lett 582(6):925–930
Park E, Lee NH, Joo HG, Jee Y (2008b) Modulation of apoptosis of eckol against ionizing radiation in mice. Biochem Biophys Res Commun 372(4):792–797
Park EJ, Ahn G, Yun JS, Kim MJ, Bing SJ, Kim DS, Lee J, Lee NH, Park JW, Jee Y (2010) Dieckol rescues mice from lethal irradiation by accelerating hemopoiesis and curtailing immunosupression. Int J Radiat Biol 88(10):848–859
Rashid K, Das J, Sil PC (2013) Taurine ameliorate alloxan induced oxidative stress and intrinsic apoptotic pathway in the hepatic tissue of diabetic rats. Food Chem Toxicol 51:317–329
Rigoulet M, Yoboue ED, Devin A (2011) Mitochondrial ROS generation and its regulation: mechanisms involved in H(2)O(2) signaling. Antioxid. Redox Signal 14:459–468
Shih CC, Wu YW, Lin WC (2002) Antihyperglycaemic and anti-oxidant properties of Anoectochilus Formosanus in diabetic rats. Clin Exp Pharmacol Physiol 29(8):684–688
Styskal J, Van Remmen H, Richardson A, Salmon AB (2012) Oxidative stress and diabetes: what can we learn about insulin resistance from antioxidant mutant mouse models? Free Radic Biol Med 52(1):46–58
Wei X, Xu J, Yang J, Liu X, Zhang R, Wang W, Yang J (2015) Involvement of a Serpin serine protease inhibitor (OoSerpin) from mollusc Octopus ocellatus in antibacterial response. Fish Shellfish Immunol 42(1):79–87
Yamazaki A, Yoshida M, Uematsu K (2002) Post-hatching development of the brain in Ocutopus ocellatus. Zoolog Sci 19(7):763–771
Zhou J, Li Y, Yan G, Bu Q, Lv L, Yang Y, Zhao J, Shao X, Deng Y, Zhu R, Zhao Y, Cen X (2011) Protective role of taurine against morphine-induced neurotoxicity in C6 cells via inhibition of oxidative stress. Neurotox Res 20(4):334–342
Acknowledgement
This research was financially supported by Basic science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2013R1A1A2064909).
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Lee, W. et al. (2017). Radio-Protective Effects of Octopus ocellatus Meat Consisted of a Plentiful Taurine Against Damages Caused by Gamma Ray Irradiation. In: Lee, DH., Schaffer, S.W., Park, E., Kim, H.W. (eds) Taurine 10. Advances in Experimental Medicine and Biology, vol 975. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1079-2_76
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DOI: https://doi.org/10.1007/978-94-024-1079-2_76
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