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Harmonic Generation Microscopy

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Book cover The Current Trends of Optics and Photonics

Part of the book series: Topics in Applied Physics ((TAP,volume 129))

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Abstract

Since the second-harmonic generation (SHG) and two-photon fluorescence (2PF) microscopies were demonstrated in 1961 (Franken et al. in Phys Rev Lett 7:118–119, 1961, [1]), two-photon microscopy has emerged as one of the most common technique used in biomedical applications. Based on the various endogenous fluorophores or extrinsic fluorescent staining, 2PF microscopy can provide cellular, morphological, and molecular information in bio-tissues (Masters et al. in Biophys J 72:2405–2412, 1997, [2], Konig in J Biophoton 1(1):13–23, 2008, [3]).

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Authors and Affiliations

  1. Department of Optics and Photonics, National Central University, Zhongli, Taiwan

    Szu-Yu Chen

  2. Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan

    Chi-Kuang Sun

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  1. Szu-Yu Chen
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  2. Chi-Kuang Sun
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Correspondence to Szu-Yu Chen .

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Editors and Affiliations

  1. Thin Film Technology Center/Dept of Optics and Photonics, National Central University, Chung-Li, Taiwan

    Cheng-Chung Lee

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Chen, SY., Sun, CK. (2015). Harmonic Generation Microscopy. In: Lee, CC. (eds) The Current Trends of Optics and Photonics. Topics in Applied Physics, vol 129. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-9392-6_29

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  • DOI: https://doi.org/10.1007/978-94-017-9392-6_29

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