Abstract
In the leaves of some species, ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco, EC 4.1.1.39) activity is substantially lower when measured following a night period than when measured after the leaves have been subjected to high light conditions for a period of time (1,2,3,). This phenomenon of predawn or dark inhibition of rubisco has been attributed to the presence of a small mol wt phosphorylated molecule which binds tightly at the enzyme’s active site (2,4) and contains 1 mol of P/mol of inhibitor (4). Binding of the inhibitor is sufficiently tight such that following purification of the enzyme most of the inhibitor remains bound (4). This fact was exploited to afford purification of the endogenous inhibitor from tobacco. Using a similar procedure the inhibitor has been purified from predawn leaves of potato and identified using NMR spectroscopy (see Gutteridge et al., these proceedings).
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Vu, C. V., Bowes, G. and Allen, L. H. (1984) Plant Physiol. 76, 843–845
Seemann, J. R., Berry, J. A., Freas, S. A. and Krump, M. A. (1985) Proc. Natl. Acad. Sci. USA 82, 8024–8028
Servaites, J. C., Parry, M. A. J., Gutteridge, S. and Keys, A. J. (1986) Plant Physiol, in press
Servaites, J. C. (1985) Plant Physiol. 78, 839–843
Wood, T. (1968) J. Chrom. 35, 352–361
Penny, C. L. (1976) Anal. Biochem. 75, 201–210
Esen, A. (1978) Anal. Biochem. 89, 264–273
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Servaites, J.C. (1987). Purification of an Endogenous Inhibitor of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. In: Biggins, J. (eds) Progress in Photosynthesis Research. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-0516-5_81
Download citation
DOI: https://doi.org/10.1007/978-94-017-0516-5_81
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-017-0518-9
Online ISBN: 978-94-017-0516-5
eBook Packages: Springer Book Archive