Abstract
Amyloid formed from islet amyloid polypeptide (lAPP) is present in pancreatic islets of spontaneously diabetic humans, monkeys and cats and in some insulinomas. The factors which cause amyloid deposition are unknown but may be related to abnormal processing or catabolism of the peptide. To determine the sites at which fibrils are formed, immunoreactivity for lAPP (lAPP-IR) was examined electron microscopically in 16 specimens of insulinoma and in pancreatic autopsy specimens from two pre-diabetic monkeys. In 9/16 insulinomas, lAPP-IR was detected on extracellular amyloid and in 6 of these, IAPP-IR was located on intracellular fibrils some of which were membrane bound. IAPP-IR was found on the radially arranged islet amyloid of pre-diabetic monkey islets. IAPP-IR on fibrils at intracellular sites suggests that amyloidosis may commence inside the cell. Subsequently, the fibrils would be extruded from the cell or become extracellular as a result of cell death.
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© 1991 Springer Science+Business Media Dordrecht
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Clark, A. et al. (1991). Intracellular Formation of Amyloid Fibrils in B-Cells of Human Insulinoma and Pre-Diabetic Monkey Islets. In: Natvig, J.B., et al. Amyloid and Amyloidosis 1990. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3284-8_113
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DOI: https://doi.org/10.1007/978-94-011-3284-8_113
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5450-8
Online ISBN: 978-94-011-3284-8
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