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Molecular Characterization of Erwinia carotovora hrpL, which Encodes an Alternate Sigma Factor

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Plant Pathogenic Bacteria

Abstract

Many plant and animal pathogenic bacteria use the type III-secretion system to deliver virulence factors into host cells. It is becoming clear that the type III-secretion systems, in phytopathogenic and plant associated bacteria, are specifically used to transport Harpins, the elicitors of hypersensitive reaction (HR), as well as avirulence gene (avr) products, disease-specific (dsp) gene products and other accessory factors that could contribute to pathogenicity (3,1,8). It is, therefore, perhaps to be expected that hrp (gene for HR & pathogenicity), avr, dsp and other genes controlling host interaction are co-regulated by common signals, transcription factors including novel sigma factors, and post-transcription regulators.

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References:

  1. Bogdanove, A. J., Kim, J. F., Wei, Z., Kolchinsky, P., Charkowski, A. O., Conlin, A. K., Collmer, A., and Beer, S. V. 1998. Homology and functional similarity of an hrp-linked pathogenicity locus, dspEF, of Erwinia amylovora and the avirulence locus avrE of Pseudomonas syringae pathovar tomato. Proc. Natl. Acad. Sci. USA 95:1325–1330.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  2. Chatterjee, A., Cui, Y., Liu, Y., Dumenyo, C. K., and Chatterjee, A. K. 1995. Inactivation of rsmA leads to overproduction of extracellular pectinases, cellulases, and proteases in Erwinia carotovora subsp. carotovora in the absence of the starvation/cell density-sensing signal, N-(3-oxohexanoyl)-L- homoserine lactone. Appl. Environ. Microbiol. 61:1959–1967.

    PubMed  CAS  PubMed Central  Google Scholar 

  3. Collmer, A., Alfano, J. R., Anderson, D. M., Badel, J. L., Deng, W.-L., Fouts, D. E., Rehm, A. H., Rojas, C. M., Schneewind, O., and van Dijk, K. 2000. Hrp (type III) protein secretion and the virulence of Pseudomonas syringae and Erwinia chrysanthemi, p. 65–70. In P. J.G. M. de Wit, T. Bisseling, and W. Stiekema (ed.), Biology of Plant-Microbe Interactions, vol. 2. International Society for Molecular Plant-Microbe Interactions, St. Paul.

    Google Scholar 

  4. Cui, Y., Madi, L., Mukherjee, A., Dumenyo, C. K., and Chatterjee, A. K. 1996. The RsmA” mutants of Erwinia carotovora subsp. carotovora strain Ecc71 overexpress hrpN Ecc and elicit a hypersensitive reaction-like response in tobacco leaves. Mol. Plant-Microbe Interact. 9: 565–573.

    Article  CAS  Google Scholar 

  5. Grimm, C., Aufsatz, W., and Panopoulos, N. J. 1995. The hrpRS locus of Pseudomonas syringae pv. phaseolicola constitutes a complex regulatory unit. Mol. Microbiol. 15:155–165.

    Article  PubMed  CAS  Google Scholar 

  6. Hendrickson, E. L., Guevera, P., and Ausubel, F. M. 2000. The alternative sigma factor RpoN is required for hrp activity in Pseudomonas syringae pv. maculicola and acts at the level of hrpL transcription. J. Bacteriol. 182:3508–3516.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  7. Huang, H. C., Lin. R. H., Chang, C. J., Collmer, A., and Deng, W. L. 1995. The complete hrp gene cluster of Pseudomonas syringae pv. syringae 61 includes two blocks of genes required for harpinPss secretion that are arranged colinearly with Yersinia ysc homologs. Mol. Plant-Microbe Interact. 8:733–746.

    Article  CAS  Google Scholar 

  8. Lindgren, P. B. 1997. The role of hrp genes during plant-bacterial interactions. Annu. Rev. Phytopathol. 35:129–152.

    Article  PubMed  CAS  Google Scholar 

  9. Mukherjee, A., Cui, Y., Liu, Y., and Chatterjee, A. K. 1997. Molecular characterization and expression of the Erwinia carotovora hrpN Ecc gene, which encodes an elicitor of the hypersensitive reaction. Mol. Plant- Microbe Interact. 10:462–71.

    Article  PubMed  CAS  Google Scholar 

  10. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual., 2nd ed. Cold Spring Habor Laboratory Press., Cold Spring Habor, N.Y.

    Google Scholar 

  11. Wei, Z. M., Beer, S. V. 1995. hrpL activates Erwinia amylovora hrp gene transcription and is a member of the ECF subfamily of sigma factors. J. Bacterid. 177:6201–6210.

    CAS  Google Scholar 

  12. Xiao, Y., S. Heu, J. Yi, Y. Lu, and S. W. Hutcheson. 1994. Identification of a putative alternate sigma factor and characterization of a multicomponent regulatory cascade controlling the expression of Pseudomonas syringae pv. syringae Pss6l hro and hrmA genes. J. Bacteriol. 176:1025–1036.

    PubMed  CAS  PubMed Central  Google Scholar 

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Acknowledgments

Our research is supported by the National Science Foundation (grant MCB-9728505) and the Food for the 21st Century Program of the University of Missouri.

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Chatterjee, A., Cui, Y., Liu, Y., Chatterjee, A.K. (2001). Molecular Characterization of Erwinia carotovora hrpL, which Encodes an Alternate Sigma Factor. In: De Boer, S.H. (eds) Plant Pathogenic Bacteria. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0003-1_54

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  • DOI: https://doi.org/10.1007/978-94-010-0003-1_54

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-3858-4

  • Online ISBN: 978-94-010-0003-1

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