Abstract
High light induces the production of cysteine and glutathione (GSH) as part of the oxidative stress response triggered by reactive oxygen species. Cysteine is produced in a two-step process, in which serine acetyltransferase (SAT) catalyses the first reaction. In Arabidopsis thaliana cyclophilin CYP20-3 was reported to promote plastidic SAT1 activity and thus cysteine and GSH production under high light conditions (Dominguez-Solis et al., Proc Natl Acad Sci USA 105:16386–16391, 2008). Challenging the loss of function mutant cyp20-3 with high light however did not reveal any hypersensitivity of the mutant questioning the function of CYP20-3 in regulation of cysteine synthesis. Although physical interaction of CYP20-3 and SAT1 could be confirmed by yeast two-hybrid analysis, plastidic cysteine synthesis induced by oxidative stress is probably regulated by mechanisms other than CYP20-3 function.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Dominguez-Solis JR, He Z, Lima A, Ting J, Buchanan BB, Luan S (2008) A cyclophilin links redox and light signals to cysteine biosynthesis and stress responses in chloroplasts. Proc Natl Acad Sci USA 105:16386–16391
Haas FH, Heeg C, Queiroz R, Bauer A, Wirtz M, Hell R (2008) Mitochondrial serine acetyltransferase functions as a pacemaker of cysteine synthesis in plant cells. Plant Physiol 148:1055–1067
Heeg C, Kruse C, Jost R, Gutensohn M, Ruppert T, Wirtz M, Hell R (2008) Analysis of the Arabidopsis O-acetylserine(thiol)lyase gene family demonstrates compartment-specific differences in the regulation of cysteine synthesis. Plant Cell 20:168–185
Massonnet C, Vile D, Fabre J, Hannah MA, Caldana C, Lisec J, Beemster GTS, Meyer RC, Messerli G, Gronlund JT, Perkovic J, Wigmore E, May S, Bevan MW, Meyer C, Rubio-Dìaz S, Weigel D, Micol JL, Buchanan-Wollaston V, Fiorani F, Walsh S, Rinn B, Gruissem W, Hilson P, Hennig L, Willmitzer L, Granier C (2010) Probing the reproducibility of leaf growth and molecular phenotypes: a comparison of three Arabidopsis accessions cultivated in ten laboratories. Plant Physiol 152:2142–2157
Muthuramalingam M, Seidel T, Laxa M, Nunes de Miranda SM, Gärtner F, Ströher E, Kandlbinder A, Dietz K-J (2009) Multiple redox and non-redox interactions define 2-Cys peroxiredoxin as a regulatory hub in the chloroplast. Mol Plant 2:1273–1288
Romano PGN, Horton P, Gray JE (2004) The Arabidopsis cyclophilin gene family. Plant Physiol 134:1268–1282
Watanabe M, Mochida K, Kato T, Tabata S, Yoshimoto N, Noji M, Saito K (2008) Comparative genomics and reverse genetics analysis reveal indispensable functions of the serine acetyltransferase gene family in Arabidopsis. Plant Cell 20:2484–2496
Wirtz M, Birke H, Heeg C, Mueller C, Hosp F, Throm C, Koenig S, Feldman-Salit A, Rippe K, Petersen G, Wade RC, Rybin V, Scheffzek K, Hell R (2010) Structure and function of the hetero-oligomeric cysteine synthase complex in plants. J Biol Chem 285:32810–32817
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media Dordrecht
About this paper
Cite this paper
Speiser, A., Kurzyk, A., Wawrzynska, A., Wirtz, M., Sirko, A., Hell, R. (2012). The Role of Cyclophilin CYP20-3 in Activation of Chloroplast Serine Acetyltransferase Under High Light Stress. In: De Kok, L., et al. Sulfur Metabolism in Plants. Proceedings of the International Plant Sulfur Workshop, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-4450-9_32
Download citation
DOI: https://doi.org/10.1007/978-94-007-4450-9_32
Published:
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-007-4449-3
Online ISBN: 978-94-007-4450-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)