Abstract
The expression products of many oncogenes is known to mediate cell proliferation [1–3], and much interest has been focused upon oncogene expression as a marker for neoplastic phenotypes [4]. Additionally, the level of expression of certain oncogenes in some tumors has been a significant indicator of biological behavior [5]. In this study, we examined the relative efficacy of immunogold silver (IGS) and peroxidase-antiperoxidase (PAP) immunohistochemistry in detecting the expression product of the epidermal growth factor receptor (EGF-R), c-myc, ras, and c-fos in 20 high-grade astrocytomas.
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Hermansson M, Nister M, Betsholtz C, et al. (1988) Endothelial cell hyperplasia in human glioblastoma: Coexpression of mRNA for platelet-derived growth factor B chain and PDGF receptor suggests autocrine growth stimilation. Proc Natl Acad Sci USA 85: 7748–7752
Libermann TA, Razon AD, Bartal Y, et al. (1984) Expression of epidermal growth factor receptors in human brain tumors. Cancer Res 44: 753–760
Moelling KB, Heimann N, Beimling P, et al. (1984) Serine and threonine-specific protein kinase activities of purified gag-mil and gag-raf proteins. Nature 312: 558–561
Thompson TC, Southgate J, Kitchener G, Land H (1989) Multistage carcinogenesis induced by ras and c-myc oncogenes in a reconstituted organ. Cell 56: 917–930
Brodeur GM, Seeger RC, Shwab M, Varmus HE, et al. (1985) Amplification of the N-myc sequences in primary human neuroblastomas: Correlation with advanced disease stage. Prog Clin Biol Res 175: 105–113
Holgate CS, Jackson P, Cowen PN, et al. (1983) Immunogold silver staining: A new method of immunostaining with enhanced sensitivity. J Histochem Cytochem 31: 983–984
Filimus J, Pollak MN, Cairncross JG, et al. (1985) Amplified, overexpressed and rearranged epidermal growth factor receptor gene in a human astrocytoma cell line. Biochem Biophys Res Commun 131: 207–215
Weinberg RA (1989) Oncogenes, antioncogenes and the molecular basis of multistep carcinogenesis. Cancer Res 49: 3713–3721
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© 1991 Springer-Verlag Tokyo
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Yoshida, S., Tanaka, R., Kaye, A.H. (1991). Oncogene Expression in High-Grade Astrocytomas: A Comparative Study of Immunogold-Silver and Peroxidase-Antiperoxidase Immunohistochemistry. In: Tabuchi, K. (eds) Biological Aspects of Brain Tumors. Springer, Tokyo. https://doi.org/10.1007/978-4-431-68150-2_49
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DOI: https://doi.org/10.1007/978-4-431-68150-2_49
Publisher Name: Springer, Tokyo
Print ISBN: 978-4-431-68152-6
Online ISBN: 978-4-431-68150-2
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