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Molecular Histochemical Techniques

  • Book
  • © 2000

Overview

Editors:
  1. Takehiko Koji

    1. Department of Histology and Cell Biology, Nagasaki University, Nagasaki, Japan

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  • Easy-to-follow step-by-step instructions for immediate use at the lab bench
  • Protocols with a uniform clear structure and layout allow the techniques to be successfully reproduced
  • All necessary practical details and notes as well as a troubleshooting section are included
  • Up-to-date methods written by experts in their fields
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (18 chapters)

  1. Front Matter

    Pages I-IX
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  2. Part I

    1. Front Matter

      Pages 1-1
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    2. Introduction to the Detection of DNA Strand Breaks

      • Takehiko Koji
      Pages 3-12

    3. In Situ Nick Translation

      • Satoshi Hashimoto, Takashi Kanematsu
      Pages 13-18

    4. In Situ Nick End-labeling: Light Microscopical

      • Shigeyoshi Oba, Takehiko Koji
      Pages 19-30

    5. In Situ Nick End-labeling:Electron Microscopical

      • Yoshinori Otsuki, Yuko Ito
      Pages 31-46

  3. Part II

    1. Front Matter

      Pages 47-47
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    2. Introduction to the Detection of Specific DNA and RNA Sequences

      • Takehiko Koji
      Pages 49-64

    3. In Situ Hybridization for DNA: Fluorescent Probe

      • Morimasa Matsuta, Mayumi Matsuta
      Pages 65-99

    4. In Situ Hybridization for RNA: Radioactive DNA Probe

      • Takashi Sawai, Miwa Uzuki
      Pages 100-114

    5. In Situ Hybridization for RNA: Radioactive RNA Probe — A Protocol for Practice With a Kit

      • Hidefumi Yoshioka, Sumihare Noji
      Pages 115-127

    6. In Situ Hybridization for RNA: Nonradioactive Probe: ds cDNA Probe

      • Hirotaka Matsumi, Tetsu Yano
      Pages 128-138

    7. In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probe

      • Yoshio Kanemitsu, Takehiko Koji
      Pages 139-152

    8. In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: T-T Dimer

      • Yoshitaka Hishikawa, Masamoto Murakami, Masao Kasahara, Naofumi Nagasue
      Pages 153-171

    9. In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (1)

      • Yuka Iwasaki, Ihachi Kato
      Pages 172-181

    10. In Situ Hybridization tor RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (II)

      • Masanobu Miyazaki, Yoshiyuki Ozono, Takashi Harada, Shigeru Kohno
      Pages 182-195

    11. In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe

      • Haruo Ohtani
      Pages 196-203

    12. Electron Microscopic in Situ Hybridization and its Combination With Immunohistochemistry

      • Akira Matsuno, Tadashi Nagashima, R. Yoshiyuki Osamura, Keiichi Watanabe
      Pages 204-221

    13. Whole Mount in Situ Hybridization for mRNA Detection in Chick Embryos

      • Hideyo Ohuchi, Sumihare Noji
      Pages 222-234

  4. Part III

    1. Front Matter

      Pages 235-235
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Keywords

About this book

Molecular Histochemical Techniques presents detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. In situ nick translation and TUNEL are described in detail, along with radioactive and nonradioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, a new development in molecular histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. Methodology is set forth step-by-step in easy-to-follow language by leading scientists who are working in the field today. The book serves as both a rich source of the latest information and a practical lab manual.

Editors and Affiliations

  • Department of Histology and Cell Biology, Nagasaki University, Nagasaki, Japan

    Takehiko Koji

Bibliographic Information

  • Book Title: Molecular Histochemical Techniques

  • Editors: Takehiko Koji

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-4-431-67915-8

  • Publisher: Springer Tokyo

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Japan 2000

  • Hardcover ISBN: 978-4-431-70231-3Published: 17 April 2000

  • Softcover ISBN: 978-4-431-68520-3Published: 22 November 2012

  • eBook ISBN: 978-4-431-67915-8Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: X, 263

  • Topics: Cell Biology, Molecular Medicine

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