Summary
We developed a model by which the transvascular removal of unnecessary substances in brain edema fluid could be measured quantitatively and chronologically. Brain stab wounds were produced in Wistar rats by insertion of paired microdialysis probes in the unilateral caudatoputamen. Homovanillic acid (HVA) was administered by microdialysis from one probe, and the HVA clearance was measured by HPLC analysis of perfusate from the other probe. Using this model, we evaluated the site of removal and whether the removal processes were affected by anesthesia or an elevated plasma concentration of the substance. As a result, 1) Probenecid did not change HVA clearance although this inhibits HVA removal via subarachnoidal vessels4. Therefore, HVA removal in this model was considered mainly due to intraparenchymal transvascular efflux. 2) There was no alteration in HVA removal induced by anesthesia or intravenous HVA injection. Consequently, this efflux mechanism seems to be a rather stable protective process, and seems to play a considerable role in brain microenvironmental homeostasis.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Aizenstein M L, Korf J (1978) Aspects of influx and efflux of homovanillic acid of rat cerebrospinal fluid. Brain Res 149:129–140
Benveniste H, Diemer N H (1987) Cellular reactions to implatation of microdialysis tube in the rat hippocampus. Acta Neuropathol (Bed) 74: 9–14
Dietzel I, Heinemann U, Hofmeier G, et al (1982) Stimulusinduced changes in extracellular Na+ and Cl−concentration in relation to changes in the size of the extracellular space. Exp Brain Res 46: 73–84
Extein I, Korf J, Roth R H, et al (1973) Accumulation of 3-rnethoxy-4-hydroxyphenylglycol-sulfate in rabbit cerebrospinal fluid following probenecid. Brain Res 54: 403–407
Fishman R A (1963) Studies of transport of sugars between blood and cerebrospinal fluid in normal states and in meningeal carcinomatosis. Trans Am Neurol Assoc 88: 114–118
Kadota E, Nonaka K, Karasuno M, et al (1993) Quantitative evaluation of unnecessary substances in brain edema fluid in rats. Adv Neurotrauma Res, in press
Lightfoote W E, Molinari G F, Chase T N (1977) Modification of cerebral ischemic damage by anesthetics. Stroke 8: 627–628
Saito Y (1971) The effect of anesthetics and hypotension on cerebral cotical blood flow. Anesth 20: 504–513 (Jpn)
Tossman U, Ungerstedt U (1986) Microdialysis in the study of extracellular levels of amino acids in the rat brain. Acta Physiol Scand 128: 9–14
Ueda T, Wakisaka S, Ochiai H et al (1991) A study about the intracerebral accumulation of thalium 201. Jpn J Cereb Blood Flow Metab 3:92 (Jpn)
Ungerstadt U, Hallstroem A (1987) In vivo microdialysis. A new approach to the analysis of neurotransmitters in the brain. Life Sci 41: 861–864
Venero J L, Machado A, Cano J (1991) Turnover of dopamine and serotonin and their metabolites in the striatum of aged rats. J Neurochem 56: 1940–1948
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer-Verlag
About this paper
Cite this paper
Kadota, E. et al. (1994). Experimental Quantitative Evaluation of Transvascular Removal of Unnecessary Substances in Brain Edema Fluid. In: Ito, U., et al. Brain Edema IX. Acta Neurochirurgica, vol 60. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9334-1_43
Download citation
DOI: https://doi.org/10.1007/978-3-7091-9334-1_43
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-9336-5
Online ISBN: 978-3-7091-9334-1
eBook Packages: Springer Book Archive