Summary
Addition of small amount of human cerebrospinal fluid (CSF) inhibited both types of MAO in monkey brain mitochondria. The specific binding of 3H-paroxetine decreased remarkably with increasing CSF volumes, while 3H-imipramine binding was slightly inhibited. Scatchard analysis of 3H-paroxetine binding in the presence and absence of CSF indicated that the inhibitory effect was associated with a decreased Bmax without an appreciable change in Kd. Addition of CSF induced an inhibition of uncompetitive 3H-5-HT uptake to monkey cerebral membranes. These results indicate that the materials in human CSF inhibit 3H-paroxetine binding, and modulate the uptake system for 5-HT.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Abraham KI, Ieni JR, Meyerson LR (1987) Purification and properties of a human plasma endogenous modulator for the platelet tricyclic binding/serotonin transport complex. Biochim Biophys Acta 923:8–21.
Angel I, Paul SM (1984) Inhibition of synaptosomal 5-3H-hydroxytryptamine uptake by endogenous factor(s) in human blood. FEBS Lett 171:280–284.
Barbaccia ML, Gandolfi O, Chuang DH, Costa E (1983) Modulation of neuronal serotonin uptake by a putative endogenous ligand of imipramine recognition sites. Proc Natl Acad Sci USA 80:5134–5138.
Barkai AI, Baron M, Kowalik S, Cooper B (1986) Inhibition of 3H-imipramine binding by plasma from depressed and normal subjects. Psychiatry Res 17:261–267.
Becker RE, Giambalvo CT, Fox RA, Macho M (1983) Endogenous inhibitors of monoamine oxidase present in human cerebrospinal fluid. Science 221:476–478.
Berrettini WH, Vogel WH (1978) Evidence for an endogenous inhibitor of platelet MAO in chronic schizophrenia. Am J Psychiatry 135:605–607.
Briley M, Langer SZ, Sette M (1982) Allosteric interaction between the 3H-imipramine binding site and the serotonin uptake mechanism. Br J Pharmacol 74:817–818.
Brusov OS, Fomenko AM, Katasonov AB (1985) Human plasma inhibitors of platelet serotonin uptake and imipramine receptor binding: extraction and heterogeneity. Biol Psychiatry 20:235–244.
Carlsson A, Fuxe K, Ungerstedt U (1968) The effect of imipramine on central 5-hydroxytryptamine neurons. J Pharm Pharmacol 20:150–151.
Davidson JRT, Mcleod MN, Turnbull CD, White HL, Feuer EJ (1980) Platelet monoamine oxidase activity and the classification of depression. Arch Gen Psychiatry 37:771–773.
Egashira T, Yamamoto T, Yamanaka Y (1984) Some interrelated properties of A and B form monoamine oxidase in monkey brain mitochondria. Jpn J Pharmacol 34:327–334.
Egashira T, Takano R, Yamanaka Y (1986) Demonstration of endogenous inhibitors of monoamine oxidase in dog cerebrospinal fluid. Jpn J Pharmacol 42:583–586.
Egashira T, Takano R, Yamanaka Y (1987) Modulation of neuronal MAO activity, 5-HT uptake and imipramine binding by endogenous substances in dog cerebrospinal fluid. Biochem Pharmacol 36:1781–1785.
Giambalvo CT (1984) Purification of endogenous modulators of monoamine oxidase from plasma. Biochem Pharmacol 33:3929–3932.
Glover V, Reveley MA, Sandier M (1980) A monoamine oxidase inhibitor in human urine. Biochem Pharmacol 29:467–470.
Habert E, Graham D, Tahraoui L, Claustre Y, Langer SZ (1985) Characterization of 3H-paroxetine binding to rat cortical membranes. Eur J Pharmacol 118:107–114.
Kinnier WJ, Chuang DM, Gwynn G, Cost E (1981) Characteristics and regulation of high affinity 3H-imipramine binding to rat hippocampal membranes. Neuropharmacology 20:411–419.
Langer SZ, Raisman R, Tahraoui L, Scatton B, Niddam R, Lee CR, Claustre Y (1984) Substituted tetrahydro-β-carbolines are possible candidates as endogenous ligand of the 3H-imipramine recognition site. Eur J Pharmacol 98:153–154.
Lindbrink P, Jonsson G, Fuxe K (1971) The effect of imipramine like drugs and antihistamine drugs on uptake mechanisms in the central noradrenaline and 5-hydroxytryptamine neurons. Neuropharmacology 10:521–536.
Lowry OH, Rosebrough NJ, Farr AL, Randal RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275.
Magnussen I, Tonderk K, Engback F (1982) Paroxetine, a potent selective longacting inhibitor of synaptosomal 5-HT uptake in mice. J Neural Transm 55:217–226.
Mellerup ET, Plenge P, Engelstoft M (1984) High affinity binding of 3H-paroxetine and 3H-imipramine to human platelet membranes. Eur J Pharmacol 96:303–309.
Mellerup ET, Plenge P, Nielsen M (1985) Size determination of binding polymers for 3H-imipramine and 3H-paroxetine in human platelet membranes. Eur J Pharmacol 106:411–413.
Miron B, Levitt M, Perlman R (1980) Low platelet monoamine oxidase activity: a possible biochemical correlate of borderline schizophrenia. Psychiatry Res 3:329–336.
Oreland L, Wiberg A, Askerg A, Traskman L, Sjostand L, Thoren P, Bertilsson L, Tybring G (1981) Platelet MAO activity and monoamine metabolites in cerebrospinal fluid in depressed and succidal patients and healthy controls. Psychiatry Res 4:21–30.
Raisman R, Briley M, Langer SZ (1979) Specific tricyclic antidepressant binding sites in rat brain. Nature 281:148–150.
Rehavi M, Ventura I, Sarne Y (1985) Demonstration of endogenous imipramine like material in rat brain. Life Sci 36:687–693.
Ross SB, Renyi AL (1975) Tricyclic antidepressant agents I. Comparison of the inhibition of the uptake of 3H-noradrenaline and 14C-5-hydroxytryptamine in slices and crude synaptosome preparations of the midbrain, hypothalamus regions of the rat brain. Acta Pharmacol Toxicol 36:382–394.
Wennogle LP, Meyerson LR (1985) Serotonin uptake inhibitors differentially modulate high affinity imipramine dissociation in human platelet membranes. Life Sci 36:1541–1550.
Yu PH, Boulton AA (1977) Activation of platelet monoamine oxidase by plasma in the human. Life Sci 25:31–36.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag
About this paper
Cite this paper
Egashira, T., Goto, S., Murayama, F., Yamanaka, Y. (1990). Inhibition of MAO activity, 3H-imipramine binding, 3H-paroxetine binding and 3H-5-HT uptake by human cerebrospinal fluid. In: Riederer, P., Youdim, M.B.H. (eds) Amine Oxidases and Their Impact on Neurobiology. Journal of Neural Transmission, vol 32. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9113-2_61
Download citation
DOI: https://doi.org/10.1007/978-3-7091-9113-2_61
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-82239-5
Online ISBN: 978-3-7091-9113-2
eBook Packages: Springer Book Archive