Abstract
Heme oxygenase-1 (HO-1) is a member of the heat shock protein family (HSP-32). It responds to thermal stress in cultured glial cells. To our knowledge, nothing is known about the expression and response of the HO-1 in cerebral ischemia. Therefore, we show here the induction of HO-1 in the brain of mice after global cerebral ischemia. HO-1-like immunoreactivity was detected at 12, 24, and 48 hours after ischemia recirculation. The HO-1-like immunoreactive cells were observed in astrocytes in the hippocampal dentate gyrus and CA1. The peak level of HO-1-like immunoreactivity was found 48 hours after the recirculation. HO-1-like immunoreactivity was observed in GFAP-positive astrocytes by use of a double immunostaining method. These results provide direct evidence for the induction and localization of HO-1 immunoreactivity in vivo in a mouse cerebral ischemia. We suggest that HO-1, produced in astrocytes after ischemia-recirculation, may directly affect neurons to protect from cell death.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Dohi K, Shioda S, Mizushima H, Homma H, Ozawa H, Nakai Y, Matsumoto K (1998) Delayed neuronal cell death and microglial cell reactivity in the CA1 region of the rat hippocampus in the cardiac arrest model. Med Electron Microsc 32: 85–93
Gelman BB (1995) Iron in CNS disease. J Neuropathol Exp Neurol 54: 477–486
Kawai K, Nitecka L, Ruetzler CA, Nagashima G, Joo F, Mies G, Nowak TS, Saito N, Lohr JM, Klatzo I (1985) Global cerebral ischemia associated with cardiac arrest in the rat: I. Dynamics of early neuronal changes. J Cereb Blood Flow Metab 12: 238–249
Koistinaho J, Miettinen S, Keinanen R, Vartiainen N, Roivainen R, Laitinen JT (1996) Lomg-term induction of haem oxygenase-1 (HSP-32) in astrocytes and microglia following transient focal brain ischemia in the rat. Eur J Neurosci 8: 2265–2272
Korpatchev WG, Lysenkov SP, Thieliz PS (1982) Modielirowanlie klinczeskoj smerti 1 postreanimationznoj bolezni u krys. Palol Fiziol Exp Ter 3: 78–80
Maines MD (1988) Heme oxigenase: function, multiplicity, regulatory mechanisms, and clinical applications. FASEB J 2: 2557–2568
Matsuoka Y, Kitamura Y, Ozaki M, Sakata M, Tsukahara T, Taniguchi T (1998) Induction of heme oxygenase-1 and major histocompatibility complex antigens in transient forebrain ischemia. J Cereb Blood Flow Metab 18: 824–832
Matsuoka Y, Ozaki M, Zhao H, Asai S, Ishikawa K, Kitamura Y (1999) Phosphorylation of c-jun and its Localization with heme oxygenase-1 and cyclooxygenase-2 in CA1 pyramidal neurons after transient forebrain ischemia. J Cereb Blood Flow Metab 19: 1247–1255
Matz P, Turner C, Weinstein PR, Massa SM, Panter SS, Sharp FR (1995) Heme-oxygenase-1 induction in glia throughout rat brain following experimental subarachnoid hemorrhage. Brain Res 713: 211–222
McCoubrey WK Jr, Huang TJ, Maines MD (1997) Isolation and characterization of a cDNA from the rat brain that encodes hemoprotein heme oxygenase-3. Eur J Biochem 247: 725–732
Paschen W, Uto A, Djuricic B, Schmitt J (1994) Heme oxygenase expression after reversible ischemia of rat brain. Neurosci Lett 180 (1): 5–8
Shibahara S, Muller RM, Taguchi H (1987) Transcriptional control of rat heme oxygenase by heat shock. J Biol Chem 262: 12889–12892
Shibahara S, Muller R, Taguchi M, Yoshida T (1985) Cloning and expression of cDNA for rat heme oxygenase. Proc Nat] Acad Sci USA 82: 7865–7869
Shioda S, Zhou CJI, Dohi K, Mizushima H, Funahashi H, Ohtaki H, Nakajo S, Arata S, Hirabayashi T, Horai R, Asano M, Iwakura Y, Takaki A (2000) Reduced postischemic apoptosis of mice deficient in interleukin-1. Soc Neurosci Abstr 30: 1810, 670.16
Singhal AB, Wang X, Sumii T, Mori T, Lo EH (2002) Effects of normobaric hyperoxia in a rat model of focal cerebral ischemiareperfusion. J Cereb Blood Flow Metab 22: 861–868
Stocker R, Glazer AN, Ames BN (1987) Antioxidant activity of albumin-bound bilirubin. Proc Natl Acad Sci USA 84: 59185922
Stocker R, Yamamoto Y, McDonagh AF, Glazer AN, Ames BN (1987) Bilirubin is an antioxidant of possible physiological importance. Science 235: 1043–1046
Takeda A, Onodera H, Sugimoto A, Itoyama Y, Kogure K, Shibahara S (1994) Increased expression of heme oxygenase mRNA in rat brain following transient forebrain ischemia. Brain Res 666: 120–124
Verma A, Hirsch DJ, Glatt CE, Ronnett GV, Snyder SH (1993) Carbon monoxide: a putative neural messenger. Science 259: 381–384
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer-Verlag Wien
About this paper
Cite this paper
Imaizumi, Y., Mizushima, H., Dohi, K., Ohtaki, H., Funahashi, H., Shioda, S. (2003). Hippocampal heme oxigenase-1 in a murine cardiac arrest model. In: Kuroiwa, T., et al. Brain Edema XII. Acta Neurochirurgica Supplements, vol 86. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0651-8_23
Download citation
DOI: https://doi.org/10.1007/978-3-7091-0651-8_23
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-7220-9
Online ISBN: 978-3-7091-0651-8
eBook Packages: Springer Book Archive