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The New Strategy of Breeding Cytidine Excessive Biosynthesis Mutants by pyr Operon Rearrangement of Bacillus amyloliquefaciens

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Advances in Applied Biotechnology

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 333))

Abstract

Cytidine is a good antitumor and antiviral intermediate which can also be used as a healthy food ingredients. With the market for cytidine increasing, the large-scale production of cytidine by microbial fermentation method has become a major way to solve this problem. While improving cytidine production, the regulation of pyr operon is essential to the excessive synthesis cytidine. However, its transcriptional regulation mechanism is unclear. The passage summarizes the regulation of de novo pyrimidine nucleotide biosynthesis (pyr genes) and the metabolic regulation mechanism of cytidine synthesis in Bacillus amyloliquefaciens. This paper makes the regulation protein pyrR as the research object, presents a series of Bacillus amyloliquefaciens pyrimidine operon transcriptional regulatory rearrangement strategy, effects the transcriptional regulation on cytidine biosynthesis, and aims to provide a theoretical basis for cytidine yielding strain.

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Acknowledgments

We thank our colleagues for critical reading of the manuscript and providing valuable suggestions. This work was supported by Chinese National Natural Science Foundation (Grant №31301542).

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Authors and Affiliations

  1. Bioengineering Laboratory, Agricultural College of Ningxia University, Yinchuan, 750021, China

    Qing Wu, Huiyan Liu, Haitian Fang, Jianguo He, Xiaoguang He & Linan Yu

Authors
  1. Qing Wu
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  2. Huiyan Liu
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  3. Haitian Fang
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  4. Jianguo He
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  5. Xiaoguang He
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  6. Linan Yu
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Corresponding author

Correspondence to Haitian Fang .

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Editors and Affiliations

  1. Tianjin University of Science and Technology, Tianjin, China

    Tong-Cun Zhang

  2. SBI ALApromo, Tokyo, Japan

    Motowo Nakajima

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Wu, Q., Liu, H., Fang, H., He, J., He, X., Yu, L. (2015). The New Strategy of Breeding Cytidine Excessive Biosynthesis Mutants by pyr Operon Rearrangement of Bacillus amyloliquefaciens . In: Zhang, TC., Nakajima, M. (eds) Advances in Applied Biotechnology. Lecture Notes in Electrical Engineering, vol 333. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-46318-5_68

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