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Intracellular Regulation of Ion Channels pp 141–151Cite as

Kinetics of a G-Protein Mediated Action on Neuronal Ca2+ Channels

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Part of the book series: NATO ASI Series ((ASIH,volume 60))

Abstract

Down regulation of Ca channels by neurotransmitters is predominant in nerve cells in contrast to the up regulation found in muscle preparations. Involvement of GTP-binding proteins in the inhibitory modulation of Ca channel is usually supported by one or more of the following observations: (1) The non-hydrolysable GTP analogue, GTPγS, reproduces the inhibitory action of external neurotransmitters (Dolphin and Scott, 1987), (2) Ca channel modulation by neurotransmitters and GTPγS is prevented by cell incubation with pertussis toxin (PTX) (Lewis et al., 1986; Toselli and Lux, 1990), (3) intracellularly applied purified Go and Gi-proteins or subunits revive the receptor mediated responsiveness in PTX-treated cells (Toselli and Lux, 1989), and (4) monoclonal antibodies against G-protein subunits prevent neurotransmitter-induced inhibition of the Ca currents (Harris-Warwick et al., 1989; McFadzean et al., 1989). The block of Ca currents predominantly affects the high voltage activated types. It is usually manifested by a slowing of the rise of current in response to a depolarizing voltage step. The current then settles to some steady state with reduced amplitudes. G-protein mediated inhibitory actions on HVA Ca currents are suggested for a variety of cells and different transmitters and drugs (Table 1).

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References

  • Bean B (1989b) Neurotransmitter inhibition of neuronal calcium currents by changes in channel voltage dependence. Nature 340: 153–156

    Article  PubMed  CAS  Google Scholar 

  • Birnbaumer L, Hildebrandt, JD, Codina J, Mattera R, Cerione RA, Sunyer T, Rojas FJ, Caron MG, Lefkowitz RJ, Lyengar, R (1985) Structural basis of adenylate cyclase stimulation and inhibition by distinct guanine nucleotide regulatory proteins. In: Molecular Mechanisms of Signal Transduction, eds P Cohen and M Houslay, 131–182

    Google Scholar 

  • Bley KR and Tsien RW (1990) Inhibition of Ca2+ and K+ channels in sympathetic neurons by neuropeptides and other ganglionic transmitters. Neuron 2: 379–391

    Article  Google Scholar 

  • Deisz RA and Lux HD (1985) 7-aminoburyric acid-induced depression of calcium currents of chick sensory neurons. Neurosci Lett 56:205–210

    Article  Google Scholar 

  • Dolphin AC, Forda SR and Scott RH (1986) Calcium-dependent currents in cultured rat dorsal root ganglion neurones are inhibited by an adenosine analogue. J Physiol 373: 47–61

    PubMed  CAS  Google Scholar 

  • Dolphin AC, Scott RH (1987) Calcium channel currents and their inhibition by (-)-Baclofen in rat sensory neurones modulation by guanine nucleotides. J Physiol 386: 670–672

    Google Scholar 

  • Dolphin AC and Scott RH (1990) Activation of calcium channel currents in rat sensory neurons by large depolarizzations. Effect of guanine nucleotides and (-)-Baclofen. Euro. J. Neurosci. 2, 104–108

    Article  CAS  Google Scholar 

  • Dunlap K and Fischbach GD (1978) Neurotransmitters decrease the calcium component of sensory neurone action potentials. Nature 276: 837–838

    Article  PubMed  CAS  Google Scholar 

  • Dunlap K and Fischbach GD (1981) Neurotransmitters decrease the calcium conductance activated by depolarization of embryonic chick sensory neurones. J Physiol 317: 519–535

    PubMed  CAS  Google Scholar 

  • Elmslie KS, Zhou W, Jones SW (1990) LHRH and GTP’S modify calcium current activation in bullfrog sympathetic neurons. Neuron 5: 75–80

    Article  PubMed  CAS  Google Scholar 

  • Ewald DA, Pang JH, Sternweis PC, Miller RJ (1989) Differential Gprotein-mediated coupling of neurotransmitter receptors to Ca2+ channels in rat dorsal root ganglion neurons in vitro. Neuron 2: 1185–1193

    Article  PubMed  CAS  Google Scholar 

  • Gahwiler BH, Brown DA (1987) Muscarine affects calcium-currents in rat hippocampal pyramidal cells in vitro. Neurosci Lett 76: 301–306

    Article  PubMed  CAS  Google Scholar 

  • Grassi F, Lux HD (1989) Voltage-dependent GABA-induced modulation of calcium currents in chick sensory neurons. Neurosci Lett 105: 113–119

    Article  PubMed  CAS  Google Scholar 

  • Gross RA, MacDonald RL (1987) Dynorphin A selectively reduces a large transient ( N-type) calcium current of mouse dorsal route ganglion neurons in cell culture. Proc natn Acad Sci USA 84: 5469–5473

    Google Scholar 

  • Harris-Warrick RM, Hammond C, Paupardin-Tritsch D, Homburger V, Rouot B, Bockaert J, Gerschenfeld HM (1989) A a40-subunit of a GTP-binding protein immunologically related to Go mediates a dopamine-induced decrease of Ca2+ current in snail neurons. Neuron 1: 27–32

    Article  Google Scholar 

  • Hescheler J, Rosenthal W, Trautwein W, Schultz G (1987) The GTPbinding protein, Go, regulates neuronal calcium channels. Nature 325: 445–447

    Article  PubMed  CAS  Google Scholar 

  • Holz GG IV, Kream RM, Spiegel A, Dunlap K (1989) G-proteins couple a-adrenergic and GABAb receptors to inhibition of peptide secretion from peripheral sensory neurons. J Neurosci 9: 657–666

    PubMed  CAS  Google Scholar 

  • Ikeda SR, Schofield GG, Weigth FF (1987) Somatostatin blocks a calcium current in acutely isolated adult rat superior cervical ganglion neurons. Neurosci Lett 81: 123–128

    Article  PubMed  CAS  Google Scholar 

  • Ikeda SR, Schofield GG (1989) Somatostatin blocks a calcium current in rat sympathetic ganglion neurones. J Physiol 409: 221–240

    PubMed  CAS  Google Scholar 

  • Kasai H, Aosaki T (1989) Modulation of Ca-channel current by an adenosine analog mediated by a GTP-binding protein in chick sensory neurons. Pflügers Arch 414: 145–149

    Article  PubMed  CAS  Google Scholar 

  • Lewis D, Weight FF, Luini A (1986) A guanine nucleotide-binding protein mediates the inhibition of voltage-dependent calcium current by somatostatin in a pituitary cell line. Proc natn Acad Sci USA 83: 9035–9039

    Article  CAS  Google Scholar 

  • Lipscombe D, Kongsamut S, Tsien RW (1989) a-Adrenergic inhibition of sympathetic neurotransmitter release mediated by modulation of N-type calcium-channel gating. Nature 340: 639–642

    Google Scholar 

  • Luini A, Lewis D, Guild S, Schofield, G, Weight, F (1986) Somatostatin, an inhibitor of ACTH secretion, decreases cytosolic free calcium and voltage-dependent calcium current in a pituitary cell line. J Neurosci 6: 3128–3132

    PubMed  CAS  Google Scholar 

  • Marchetti C, Robello M (1989) Guanosine-5’-0-(3-thiotriphosphate) modifies kinetics of voltage-dependent calcium current in chick sensory neurons. Biophys J 56: 1267–1272

    Article  PubMed  CAS  Google Scholar 

  • McFadzean I, Docherty RJ (1989) On the mechanism by which enkephalin and noradrenaline inhibit voltage-dependent calcium currents recorded from NG108–15 hybrid cells. Eur J Neurosci 1: 141–147

    Article  PubMed  Google Scholar 

  • McFadzean I, Mullaney I, Brown DA, Milligan G (1989) Antibodies to the GTP binding protein, Go, antagonize noradrenaline-induced calcium current inhibition in NG108–15 hybrid cells. Neuron 3: 177–182

    Article  PubMed  CAS  Google Scholar 

  • Pennington NJ, Kelly JS (1990) Serotonin receptor activation reduces calcium current in an acutely dissociated adult central neuron. Neuron 4: 751–758

    Article  Google Scholar 

  • Plummer MR, Logothetis DE, Hess P (1989) Elementary properties and pharmacological sensitivities of calcium channels in mammalian peripheral neurons. Neuron 2: 1453–1463

    Article  PubMed  CAS  Google Scholar 

  • Sah DWY (1990) Neurotransmitter modulation of calcium current in rat spinal cord neurons. J Neurosci 10: 136–141

    PubMed  CAS  Google Scholar 

  • Tokutomi N and Lux HD, unpublished

    Google Scholar 

  • Toselli M and Lux HD (1989) GTP-binding proteins mediate acetylcholine inhibition of voltage dependent calcium channels in hippocampal neurons. Pflügers Arch 413: 319–321

    Article  PubMed  CAS  Google Scholar 

  • Toselli M, Lang J, Costa T, Lux HD (1989) Direct modulation of voltage-dependent calcium channels by muscarinic activation of a pertussis toxin-sensitive G-protein in hippocampal neurons. Pflügers Arch 415: 255–261

    Article  PubMed  CAS  Google Scholar 

  • Tsien RW, Lipscombe DV, Madison KR, Bley KR, Fox AP (1988) Multiple types of neuronal calcium channels and their selective modulation. Trends Neurosci 11: 431–438

    Article  PubMed  CAS  Google Scholar 

  • Tsunoo A, Yoshii M, Narahashi T (1986) Block of calcium channels by enkephalin and somatostatin in neuroblastoma x glioma hybrid NG108–15 cells. Proc natn Acad Sci USA 83: 9832–9836

    Article  CAS  Google Scholar 

  • Wanke E, Ferroni A, Malgaroli A, Ambrosini A, Pozzan T, Meldolesi J (1987) Activation of a muscarinic receptor selectively inhibits a rapidly inactivated Ca2+ current in rat sympathetic neurons. Proc natn Acad Sci USA 84: 4313–4317

    Article  CAS  Google Scholar 

  • Yatani A., Okabe K, Polakis P, Halenbeck R, McCormick F, Brown AM (1990) Ras P21 and GAP inhibit coupling of muscarinic receptors to atrial K+ channels. Cell 61, 769–776.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institute for Psychiatry, Dept. of Neurophysiology, Am Klopferspitz 18a, 8033, Planegg-Martinsried, Germany

    H. D. Lux, N. Tokutomi & F. Grassi

  2. Dept. of Neurophysiology, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, Sendai, Japan

    N. Tokutomi

  3. Dipartimento di Medicina Sperimentale, Universita dell’Aquila, I-67100, L’Aquila, Italy

    F. Grassi

Authors
  1. H. D. Lux
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  2. N. Tokutomi
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  3. F. Grassi
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Editor information

Editors and Affiliations

  1. School of Medicine, Dept. of Physiology, University of Pennsylvania, 19104-6085, Philadelphia, PA, USA

    Martin Morad  & Zalman Agus  & 

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© 1992 Springer-Verlag Berlin Heidelberg

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Lux, H.D., Tokutomi, N., Grassi, F. (1992). Kinetics of a G-Protein Mediated Action on Neuronal Ca2+ Channels. In: Morad, M., Agus, Z. (eds) Intracellular Regulation of Ion Channels. NATO ASI Series, vol 60. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84628-1_16

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  • DOI: https://doi.org/10.1007/978-3-642-84628-1_16

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84630-4

  • Online ISBN: 978-3-642-84628-1

  • eBook Packages: Springer Book Archive

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