Abstract
The genus Populus belongs to the family Salicaceae and includes 34 species which are interspersed throughout the forests of the temperate and cold regions of the northern hemisphere. Poplars are in general dioecious plants, with male and female flowers on separate individuals. Their chromosome number is 2n = 38. They are fast-growing hardwood trees (Fig. 1), which can be raised either in pure or mixed plantations, or in association with agricultural crops. Because of their rapid rate of growth, selected clones can be economically grown in short rotations, in Italy around 12 years.
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References
Ahuja MR (1981) Tissue culture technology in clonal propagation of aspen (Populus) genotypes. In: IUFRO Proc Coll Int Culture in vitro essences For, Fontainebleau, AFOCEL, Nangis, pp 315–338
Ahuja MR (1983a) Somatic cell differentiation and rapid clonal propagation of aspen. Silv Genet 32: 131–135
Ahuja MR (1983b) Short note: isolation and culture of mega and normal protoplasts in aspen. Silv Genet 32: 225–227
Ahuja MR (1984a) Protoplast research in woody plants. Silv Genet 33: 32–37
Ahuja MR (1984b) Short note: a commercially feasible micropropagation method for aspen. Silv Genet 33: 174–176
Ahuja MR (1986) Aspen. In: Evans DA, Sharp WR, Ammirato PV (eds) Handbook of plant cell culture, vol 4. Techniques and applications. MacMillan, New York, pp 626–651
Ahuja MR (1987) In vitro propagation of poplar and aspen. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry, vol 3. Case histories: gymnosperms, angiosperms and palms. Nijhoff, Dordrecht, pp 207–223
Ahuja MR, Muhs HJ (1982) Control of growth and differentiation in tissues and protoplast-derived callus in different genotypes of aspen. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 117–118
Anon (1975) Induction of haploid poplar plants from anther culture in vitro. Sci Sin 18: 769–777
Anon (1976) Haploid plants of poplar induced from anther cultured in vitro. Acta Genet Sin 3: 145–149
Anon (1977) Induction of haploid poplar plantlets from pollen. Acta Genet Sin 4: 49–54
Avanzo E, Bisoffi S, Gras MA, Mughini G (1985) Breeding strategy adopted in Italy for poplars of the Aigeiros section. Genet Agric 39: 308 (Abstr)
Bagnaresi U, Minotta G (1983) Moltiplicazione in vitro di pioppi ibridi della Sez. Leuce. Monti Boschi 2: 57–60
Barocka KH, Baus M, Lontke E, Sievert F (1985) Tissue culture as a tool for in vitro mass propagation of aspen. Pflanzenzüchtung 94: 340–343
Bawa KS, Stettier RF (1972) Organ culture with black cottonwood: morphogenetic response of female catkin primordia. Can J Bot 50: 1627–1631
Berbee FM, Berbee JG, Hildebrandt AC (1972) Induction of callus and trees from stem tip cultures of a hybrid poplar. In vitro 7: 269
Blackwell SJ, Laetsch WM, Hyde BB (1969) Development of chloroplast fine structure in aspen tissue culture. Am J Bot 56: 457–463
Blanarikova V, Karacsonyi S (1978) The isolation of tissue culture of Populus alba L. Pyramidalis. Biol Plant 20: 14–18
Brand R, Venverloo CJ (1973) The formation of adventitious organs. II. The origin of buds formed on young adventitious roots of Populus nigra L. Italica. Acta Bot Neerl 22: 399–406
Braun HJ, Schlenker G (1964) Das Vorkommen von Wurzelkeimen in den Sprossachsen verschiedener Populus-Sorten. Mitt Ver Forstl Standortskd Forstpflanzenziicht 14: 65–68
Brown CL, Sommer HE (1982) Vegetative propagation of dicotyledonous trees. In: Bonga JM, Durzan DJ (eds) Tissue culture in forestry. Nijhoff, Dortrecht, pp 109–149
Chalupa V (1974) Control of shoot formation and production of trees from poplar callus. Biol Plant 16: 316–320
Chalupa V (1975) Induction of organogenesis in forest tree tissue cultures. Commun Inst For Cech 9: 39–50
Chalupa V (1977) The use of regenerants from tissue culture of forest trees in tree breeding In: Novak FJ (ed) Use of tissue cultures in plant breeding. Olomouc Czech Acad Sei, Prague, pp 183–193
Chalupa V (1979) In vitro propagation of some broad-leaved forest trees. Commun Inst For Czech 11: 159–170
Chalupa V (1981) Clonal propagation of broad-leaved forest trees in vitro. Commun Inst For Cech 12: 255–271
Cheema GS (1987a) Poplar propagation from somatic embryoids. In: Int Worksh Agrofor Rur Needs, Vigyan Bhawan, New Delhi
Cheema GS (1987b) Isolation and culture of protoplasts from totipotent cell cultures of Populus ciliata. In: 7th Int Protoplast Symp Wageningen
Cheema GS (1989) Somatic embryogenesis and plant regeneration from cell suspension and tissue cultures of mature Himalayan poplar (Populus ciliata). Plant Cell Rep 8: 124–127
Christie, CB (1978) Rapid propagation of aspen and silver poplars using tissue culture techniques. Proc Int Plant Propagators Soc 28: 255–260
Chun YW, Hall RB, Sthephens LC (1986) Influences of medium consistency and shoot density on in vitro shoot proliferation of Populus alba x P. grandidentata. Plant Cell Tissue Organ Cult 5: 179–185
Coleman GD, Ernst SG (1989) In vitro shoot regeneration of Populus deltoids: effect of cytokinin and genotype. Plant Cell Rep 8: 459–462
Diaz-Colon JD, Bovey RW, Davis FS, Baur JR (1972) Comparative effects of concentration of picloram, 2,4,5-T and dicamba in tissue culture. Physiol Plant 18: 386–402
Douglas GC (1982) Protoplast isolation from totipotent cell-cultures of Populus hybrid TT32. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 605–606
Douglas GC (1984) Formation of adventitious buds in stem internodes of Populus spp. cultured in vitro on basal medium: influence of endogenous properties of explants. J Plant Physiol 116: 313–321
Douglas GC (1985) Formation of adventitious buds in stem internodes of Populus hybrid TT32 cultured in vitro: effects of sucrose, zeatin, IAA and ABA. J Plant Physiol 121: 225–231
Douglas GC (1989) Poplar (Populus spp.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 5: Trees II. Springer, Berlin. Heidelberg New York, pp 300–323
Durzan DJ (1982) Cell and tissue culture in forest industry. In Bonga JM, Durzan DJ (eds) Tissue culture in forestry. Nijhoff, Dordrecht, pp 36–71
Durzan DJ (1985) Tissue culture and improvement of woody perennials: an overview. In: Henke RR, Hughes KW, Constantin MJ, Hollaender A (eds) Tissue culture in forestry and agriculture. Plenum, New York, pp 233–256
Durzan DJ, Campbell RA (1974) Prospects for the mass production of improved stock of forest trees by cell and tissue culture. Can J For Res 4: 151–174
Evers PW (1983) Tree physiology and micropropagation in the People’s Republic of China: report of a visit, June 5-19. Rijksinst Onderzock Bosen Landschapsobown Dorschkamp Wageningen Rapp 350: 16 pp
FAO (ed) (1980) Poplars and willows in wood production and land use. FAO, For Ser 10
Fillatti J J, Haissig B, McCown B, Comai L, Riemenschneider D (1988) Development of glyphosate-tolerant Populus plants through expression of a mutant aro A gene from Salmonella typhimurium. In: Hanover JW, Keathley DE (eds). Genetic manipulation of woody plants. Plenum, New York, pp 243–249
Fröhlich HJ (1982) Fortschritte bei der vegetativen Vermehrung. Forstarchiv 53: 3–9
Fröhlich HJ, Weisgerber H (1985) Research on in vitro techniques within the framework of poplar breeding results and future trends. Silv Genet 34: 132–137
Gautheret RJ (1934) Culture du tissu cambial. CR Acad Sei Paris Ser D 198: 2195–2196
Ghugale DD, Kulkarni DD, Narasimhan R (1971) Effect of auxins and gibberellic acid on growth and differentiation of Morus alba and Populus nigra tissues in vitro. Indian J Exp Biol 9: 381–384
Giordano E (1959) II Populus deltoides Bartr. nel suo paese di origine. Nota preliminare su un viaggio di studio. Publ Cent Sper Agric For 3: 127–180
Giordano E (1962) Recent work on Populus deltoides in Italy. In: Proc 5th World For Congr 1960 Seattle, pp 811–812
Graham LL, Johnson MA (1982) Sucrose synthetase from triploid quaking aspen callus. Phytochemistry 17: 1231–1233
Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculetum (tomato). Planta 107: 161–170
Hisajima S, Arai Y, Thorpe TA (1985) Sucrose synthesis in callus cultures. Biol Plant 27: 74–77
Hoffman A, Miller AR, Pengelly WL (1985) Characterization of polyphenols in cell walls of cultured Populus trichocarpa tissues. Phytochemistry 24: 2685–2687
Jacquiot C (1966) Plant tissues and excised organs cultures and their significance in forest research. J Inst Wood Sci 16: 22–34
Kechel HG, Bodes E (1985) Resistenzprüfung an Pappeln aus Gewebekultur. Eur J For Pathol 15: 45–51
Kim JH, Shim SY, Noh EW, Park JI (1982) Mass production of selected poplar clones through bud culture. Res Rep Inst For Genet 18: 80–85
Kim JH, Noh EW, Park JI (1983) Haploid plantlets formation through anther culture of Populus. Res Rep Inst For Genet 19: 93–98
Kim JH, Moon HK, Park JI (1986) Plantlet regeneration on callus derived from internodal tissue of Populus alba x P. glandulosa. Res Rep Inst For Genet 22: 122–127
Kirby EG (1982) The use of in vitro techniques for genetic modifications of forest trees. In Bonga JM, Durzan DJ (eds) Tissue culture in forestry. Nijhoff, Dordrecht, pp 369–386
Kouider M, Skirvin RM, Saladin KP, Dawson JO, Jokela J J (1984) A method to culture immature embryos of Populus deltoides in vitro. Can J For Res 14: 956–958
Lee JS, Lee SK, Jang SS, Lee J J (1987) Plantlet regeneration from callus protoplasts of Populus nigra. Res Rep Inst For Genet 23: 143–148
Leshkovtseva II (1967) Formation of sporogenous cells in the culture of isolated male flower buds of woody plants (effect of heteroauxin and kinetin). Sov Plant Physiol 14: 599–605
Lester DT, Berbee JG (1977) Within clone variation among black poplar trees derived from callus culture. For Sci 23: 122–131
Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100–127
Lubrano L (1981) Propagation du peuplier. In: IUFRO Proc Coll Int Culture in vitro essences For, Fontainebleau, AFOCEL, Nangis, pp 81–86
Lubrano L (1989) Micropropagazione di Populus deltoides x P. maximowiczii Eridano. Centro Sper Agric For/Ist Sper Pioppic, Quad Ric 25, SAF, Roma Mathes MC (1964a) The culture of isolated aspen tissue. For Sci 10: 35–38
Mathes MC (1964b) The in vitro formation of plantlets from isolated aspen tissue. Phyton 21: 137–141
Matsumoto T, Nishida K, Noguchi M, Tamaki E (1973) Some factors affecting the anthocyanin formation by Populus cells in suspension culture. Agric Biol Chem 37: 561–567
Mehra PN, Cheema GS (1980) Clonal multiplication in vitro of Himalayan poplar (Populus ciliata). Phytomorphology 30: 336–343
Mehra PN, Cheema GS (1985) Differential response of male and female Himalayan poplar (Populus ciliata) and P. alba in vitro. Phytomorphology 35: 151–154
Meiden HA Van der (1975) Evolution du marché du bois de peuplier Résultats d’une enquête internationale. FAO/CIP/75/5, Rome
Michler CH, Haissig BE (1988) Increased herbicide tolerance of in vitro selected hybrid poplar. In: Ahuja MR (ed) Somatic cell genetics of woody plants. Kluwer, Dordrecht, pp 183–189
Muhle-Larsen C (1970) Recent advances in poplar breeding. In: Romberger JA, Mikola P (eds) Int Rev For Res 3. Academic Press, New York London, pp 1–67
Murashige T (1974) Plant propagation through tissue culture. Annu Rev Plant Physiol 25: 135–166
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Nkanka B (1981) Obtention de plantules à partir de bourgeons adventifs sur feuilles de peuplier (Populus x euramericana Serotina de Champagne et P. Nigra 1214) cultivés in vitro. In: IUFRO Proc Coli Int Culture in vitro essences For, Fontainebleau, AFOCEL, Nangis, pp 236–245
Parsons TS, Sinkar VP, Stettier RF, Nester EW, Gordon MP (1986) Transformation of poplar by Agrobacterium tumefaciens. Biotechnology 4: 533–536
Pelet F, Hildebrandt AC, Riker A J, Skoog F (1960) Growth in vitro of tissues isolated from normal stems and insect galls. Am J Bot 47: 186–195
Phythoud F, Sinkar VP, Nester EW, Gordon MT (1987) Increased virulence of Agrobacterium rhizogenes conferred by the vir region of pTiBo542: application to genetic engineering of poplar. Biotechnology 5: 1323–1327
Rao AN, Lee SK (1982) Importance of tissue culture in tree propagation. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 715–718
Russell J A, McCown BH (1986) Culture and regeneration of Populus leaf protoplasts isolated from non seedling tissue. Plant Sci 46: 133–142
Russell J A, McCown BH (1988) Recovery of plants from leaf protoplasts of hybrid-poplar and aspen clones. Plant Cell Rep 7: 59–62
Rutledge CB, Douglas GC (1988) Culture of meristem tips and micropropagation of 12 commercial clones of poplar in vitro. Physiol Plant 72: 367–373
Saito A (1980a) Effects of inorganic elements in the medium on shoot differentiation from Populus callus. J Jpn For Soc 62: 147–149
Saito A (1980b) Medium for shoot formation from somatic callus tissue in Populus. J Jpn For Soc 62: 270–272
Saito A (1980c) Isolation of protoplasts from mesophyll cells of Paulownia and Populus. Bull For Prod Res Inst 309: 1–6
Saito A (1980d) Fusion of protoplasts isolated from somatic cells of tree species. Bull For Prod Res Inst 309: 7–12
Sato T (1974) Callus induction and organ differentiation in anther culture of poplars. J Jpn For Soc 56: 55–62
Son SH, Hall RB (1990) Multiple shoot regeneration from root organ cultures of Populus albax P. grandidentata. Plant Cell Tissue Organ Cult 20: 53–57
Stoehr MU, Zsuffa L (1990) Induction of haploids in Populus maximowiczii via embryogenic callus. Plant Cell Tissue Organ Cult 23: 49–58
Sussex IM, Clutter ME (1959) Seasonal growth periodicity of tissue explants from woody perennial plants in vitro. Science 129: 836–837
Uddin MR, Meyer MM Jr, Jokela JJ (1988) Plantlet production from anthers of Eastern cottonwood (Populus deltoides). Can J For Res 18: 937–941
Venverloo CJ (1973) The formation of adventitious organs. I Cytokinin-induced formation of leaves and shoots in callus cultures of Populus nigra Italica. Acta Bot Neerl 22: 390–398
Verma DC, Wann SR (1983) Isolation of high yields of viable protoplasts from quaking aspen seedlings and cultured loblolly pine cell suspensions. In: Potrykus I, Harms CT, Hinnen A, Huetter R, King J, Shillitto RD (eds) 6th Int Protoplast Symp Basel. Exp Suppl 45: 10–11
Wang CC, Chu ZC, Sun CS (1975) The induction of Populus pollen plants. Acta Bot Sin 17: 56–59
Wann SR, Einspahr DW (1986) Reliable plantlet formation from seedling explants of Populus tremuloides (Michx). Silv Genet 35: 19–24
Whitehead HCM, Giles KL (1977) Rapid propagation of poplars by tissue culture methods. NZJ For Sci 7: 40–43
Winton LL (1968a) The rooting of liquid-grown aspen callus. Am J Bot 55: 159–167
Winton LL (1968b) Initiation of friable aspen callus under different light environments. Phyton 25: 23–28
Winton LL (1968c) Plantlets from aspen tissue cultures. Science 160: 1234–1235
Winton LL (1970) Shoot and tree production from aspen tissue cultures. Am J Bot 57: 904–909
Winton LL (1971) Tissue culture propagation of European aspen. For Sci 17: 348–350
Winton LL, Parham RA, Johnson MA, Einspahr DW (1974) Tree improvement by callus, cell and protoplast culture. Tappi 57 (12): 151–152
Wolter KE (1968) Root and shoot initiation in aspen callus cultures. Nature (London) 219: 509–510
Wolter KE, Gordon JC (1975) Peroxidases as indicators of growth and differentiation in aspen callus cultures. Physiol Plant 33: 219–223
Wolter KE, Skoog F (1966) Nutritional requirements of Fraxinus callus cultures. Am J Bot 53: 263–269
Wu K, Xu M (1984) Induction of maternal haploid plants from unpollinated ovaries of poplar in vitro. Acta Genet Sin 11: 51–60
Yang Y, Wang S, Zeng S, Jing Y (1980) The ways of poplar pollen development and the effect of some factors in vitro. Sci Silv Sin 16: 257–263
Youn Y, Lee JS, Lee SK (1985) Isolation and culture of protoplasts from suspension-cultured cells of Populus alba x P. glandulosa F1. Res Rep Inst For Genet 21: 109–11
Zavitkovski J (1979) Energy production in irrigated, intensively cultured plantations of Populus Tristis no. 1 and jack pine. For Sci 25: 383–392
Zhu X, Wang R, Liang Y (1980) Induction of poplar pollen plantlets. Sci Silv Sin 16: 190–197
Zimmerman RH (1985) Application of tissue culture propagation to woody plants. In: Henke RR, Hughes KW, Constantin MJ, Hollaender A (eds) Tissue culture in forestry and agriculture. Plenum, New York, pp 165–177
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Lubrano, L. (1992). Micropropagation of Poplars (Populus spp.). In: Bajaj, Y.P.S. (eds) High-Tech and Micropropagation II. Biotechnology in Agriculture and Forestry, vol 18. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76422-6_8
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