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Viral Expression Vectors pp 1–24Cite as

Retroviral Vectors

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Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 158))

Abstract

An understanding of the retroviral life cycle and the ability to manipulate the viral genome has allowed the development of gene transfer vectors which utilize the efficient gene transfer capability that retroviruses have evolved. The advantages of currently available retroviral vectors include the ability to efficiently infect a wide range of cell types from different animal species, the precise integration of genetic material carried by the vector into recipient cells, the lack of vector spread or production of viral proteins after infection, and the lack of toxicity of these viruses in infected cells. In this review I will cover features of retrovirus biology relevant to retroviral vector use, different approaches to constructing vectors, and some of the uses for retroviral vectors. Although a great number of individual vectors and virus producing cell lines are currently available, I hope to summarize the important principles to allow their use by those unfamiliar with this gene transfer technique.

This work was supported by grants from the National Heart Lung and Blood Institute and the National Cancer Institute of the National Institutes of Health.

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Authors and Affiliations

  1. Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, WA, 98104, USA

    A. D. Miller

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  1. Department of Microbiology School of Medicine, State University of New York at Stony Brook, Stony Brook, N.Y., 11794-8621, USA

    Nicholas Muzyczka Ph.D. (Associate Professor) (Associate Professor)

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© 1992 Springer-Verlag Berlin·Heidelberg

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Miller, A.D. (1992). Retroviral Vectors. In: Muzyczka, N. (eds) Viral Expression Vectors. Current Topics in Microbiology and Immunology, vol 158. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-75608-5_1

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  • DOI: https://doi.org/10.1007/978-3-642-75608-5_1

  • Publisher Name: Springer, Berlin, Heidelberg

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