Abstract
Studies were undertaken to characterize specific pathophysiological defects in the preleukemic disorders in light of our present understanding of the humoral and cellular regulation of normal granulopoiesis. Studies were conducted in blast crisis of chronic myelogenous leukemia (CML), Fanconi’s anemia, aplastic anemia, and preleukemia (as characterized by a mildly megaloblastic, packed marrow and peripheral pancytopenia). Peripheral blood and bone marrow cells from such patients were studied for their ability to produce and respond to humoral regulatory factors using the semi-solid colony forming assay system in agar-gel. These studies indicate that the defect in blast crisis of CML may involve varying mechanisms in different patients, the end result of which is always a rapidly accumulating population of immature cells. In Franconi’s anemia a specific defect in the production of granulocyte colony stimulating factor has been found. In aplastic anemia our studies indicate that there is a loss of bone marrow stem cells with retention of normal production of humoral regulators. Preleukemia, on the basis of the studies reported here, appears to involve defects in both colony stimulating activity production and stem cell responsiveness to colony stimulating factors.
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References
Bolin, R.W., Robinson, W.A.: Bacterial, serum and cellular modulation of granulopoietic activity. J. Cell Physiol. 92, 145–154 (1977)
Burgess, A.W., Wilson, E.M.A., Metcalf, D.: Stimulation by human placental conditioned medium of hemopoietic colony formation by human marrow cells. Blood 49, 573–585 (1977)
Chervenick, P.A., Ellis, L.D., Pan, S.F., Lawson, A.L.: Human leukemic cells: In vitro growth of colonies containing the Philadelphia (Ph1) chromosome. Science 174, 1134–1135 (1971)
Gatti, R.A., Robinson, W.A., Deinhard, A.S., Nesbit, M., McCullough, J.J., Ballow, M., Good, R.A.: Cyclic leukocytosis in chronic myelogenous leukemia: new perspectives on pathogenesis and therapy. Blood 41, 771–782 (1973)
Golde, D.W., Burgaleta, C., Sparkes, R.S., Cline, M.J.: The Philadelphia chromosome in human macrophage. Blood 49, 367 (1977)
Greenberg, P.L., Nichols, W.C., Schrier, S.L.: Granulopoiesis in acute myeloid leukemia and preleukemia. N. Engl. J. Med. 284, 1225–1232 (1971)
Kurnick, J.E., Robinson, W.A., Dickey, C.A.: In vitro granulocytic colony-forming potential of bone marrow from patients with granulocytic and aplastic anemia. Proc. Soc. Exp. Biol. Med. 137, 917–920 (1971)
McCaffrey, R., Harrison, T.A., Parkman, R., Baltimore, D.: Terminal transferase activity in leukemic cells and normal thymocytes. N. Engl. J. Med. 292, 775 (1975)
Moore, M., Robinson, W.A.: Granulopoietic activity of urine and cells from patients with chronic granulocytic leukemia. Proc. Soc. Exp. Biol. Med. 146, 499–503 (1974)
Moore, M.A.S., Spitzer, G., Williams, N., Metcalf, D., Buckley, J.: Agar culture studies in 127 cases of untreated acute leukemia: The prognostic value of reclassification of leukemia according to in vitro growth characteristics. Blood 44, 1–18 (1974)
Myers, A.M., Jr., Robinson, W.A.: Colony stimulating factor levels in human serum and urine following chemotherapy. Proc. Soc. Exp. Biol. Med. 148, 694–700 (1975)
Olofsson, T., Olsson, I.: Granulopoiesis in chronic myeloid leukemia. Blood 48, 351–360(1976)
Pike, B.L., Robinson, W.A.: Human bone marrow colony growth in agar-gel. J. Cell. Physiol. 76, 77–84(1970)
Quesenberry, P., Morley, A., Miller, M., Rickard, K., Howard, D., Stohlmann, F.: Effect of endotoxin on granulopoiesis and the in vitro colony-forming cell. Blood 41, 391–398 (1973)
Rhodes, C.A., Robinson, W.A., Entringer, M.A.: Granulocyte colony formation in chronic granulocytic leukemia during stable, accelerated and blastic disease. Proc. Soc. Exp. Biol. Med. 157, 337–341 (1978)
Robinson, W.A., Entringer, M.A., Bolin, R.W., Stonington, O.G., Jr.: Bacterial stimulation and granulocyte inhibition of granulopoietic factor production. N. Engl. J. Med. 297, 1129–1134(1977)
Stephenson, J.R., Axelrad, A.A., McLeod, D.L., Shreeve, M.M.: Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proc. Natl. Acad. Sci. USA 68, 1542–1546 (1971)
Stohlman, F., Jr., Quesenberry, P., Tyler, W.S.: The regulation of myelopoiesis as approached with in vivo and in vitro techniques. Prog. Hematol. 8, 259 (1973)
Williams, N., Jackson, H., Sheridan, A,P.C., Murphy, M.J., Jr., Elste, A., Moore, M.A.S.: Regulation of megakaryopoiesis in long-term murine bone marrow cultures. Blood 51, 245–255 (1978)
Wilson, J.D., Dalton, G.: Human T lymphocyte colonies in agar: a comparison with other T cell assays in healthy subjects and cancer patients. Aust. J. Exp. Biol. Med. Sci. 54, 27–34(1976)
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© 1979 Springer-Verlag Berlin Heidelberg
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Robinson, W.A., Bolin, R., Rhodes, C.A. (1979). In Vitro Disorders of Granulopoiesis in Preleukemia. In: Schmalzl, F., Hellriegel, KP. (eds) Preleukemia. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-67470-9_19
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DOI: https://doi.org/10.1007/978-3-642-67470-9_19
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-09698-6
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