Abstract
At present only one principle for enzyme reactions responsible for synthesis of polynucleotide chains is known. It consists of extension of chains in the 5’→3’ direction by a nucleophilic attack of the 3’-0H group of the 3’-terminal nucleotidyl group on the phosphorus atom of the α-phosphate group of a nucleoside di- or triphosphate, resulting in extension of the chain by a further nucleoside monophosphate. The incorporation into a polymer by this type of reaction of a nucleoside monophosphate lacking the free 3’-OH group in the ribose configuration would prevent further chain elongation since no further 3’, 5’-phosphodiester linkage can be formed. This expectation has been experimentally confirmed. Any nucleoside or nucleotide containing this type of sugar moiety could, therefore, be regarded as a potential polynucleotide chain growth terminator, and will be the topic of the present chapter.
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Frederiksen, S., Klenow, H. (1975). 3’-Deoxyadenosine and Other Polynucleotide Chain Terminators. In: Sartorelli, A.C., Johns, D.G. (eds) Antineoplastic and Immunosuppressive Agents. Handbuch der experimentellen Pharmakologie / Handbook of Experimental Pharmacology, vol 38 / 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-65806-8_37
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