Abstract
The differential display-polymerase chain reaction (DDRT-PCR) technique is a powerful method for detecting differentially expressed genes (Liang and Pardee 1992). Nonetheless, DDRT-PCR, with about 300 different primer combinations, typically results in about 50–100 differentially displayed bands, of which about 10%–40% may be artifacts. The number of artifacts can be reduced by including several identical RNA samples; however, because of the high number of artifacts it is essential to verify the differential expression. This can be done by several different quantitative or semi-quantitative approaches, e.g., northern blotting, nuclear run-on assay, quantitative PCR and RNase protection.
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Hummel, R., Jørgensen, M., Bévort, M., Grønborg, M., Leffers, H. (1997). Verification of Differential Display Results by RNase Protection. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_34
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DOI: https://doi.org/10.1007/978-3-642-60441-6_34
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