Abstract
In recent years, the most significant development in the field of synthetic chemistry has been the application of biological systems to chemical reactions. Reactions catalyzed by enzymes or enzyme systems display far greater specificities than more conventional forms of organic reactions; some have been shown to be useful for synthetic or biotechnological applications.
d-Pantoyl lactone and d-pantoic acid are important chiral building blocks for the chemical synthesis of d-pantothenic acid. We found that Candida parapsilosis produced a carbonyl reductase which converts ketopantoyl lactone to d-pantoyl lactone. Similarly, conversion of ketopantoic acid to d-pantoic acid can be carried out in a better yield with the ketopantoic acid reductase of Agrobacterium radiobacter. Optical resolution of unmodified DL-pantoyl lactone can be carried out with a novel fungal enzyme which specifically hydrolyzes d-pantoyl lactone to d-pantoic acid. The enzyme catalyzing this stereospecific hydrolysis has been shown to be a new lactonohydrolase. These enzymes may be applicable to the industrial production of d-pantothenic acid.
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© 1993 Springer-Verlag Berlin Heidelberg
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Yamada, H., Shimizu, S. (1993). Synthesis of Chiral Intermediates for D-Pantothenate Production by Microbial Enzymes. In: Yoshida, T., Tanner, R.D. (eds) Bioproducts and Bioprocesses 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-49360-7_14
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DOI: https://doi.org/10.1007/978-3-642-49360-7_14
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