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Electrochemical Analysis of Proteins and Cells pp 5–18Cite as

Theoretical Background of Electrochemical Analysis

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Part of the book series: SpringerBriefs in Molecular Science ((BRIEFSMOLECULAR))

Abstract

An electrode is a conductor or semiconductor, which directly contacts the electrolyte solution. In an electrochemical system, the input and output are both realized through an electrode. The substrate materials of the commonly used electrodes include noble metals (platinum, gold, silver, etc.), mercury, various kinds of carbon materials and semiconductor materials. Since the electron transfer rate between proteins and electrode surfaces is usually prohibitively slow, due to the burying of the electroactive prosthetic groups of most proteins in the electrically insulated peptide backbones and adsorptive denaturation of proteins on electrode surface, chemically modified electrodes (CMEs) are developed to facilitate the electrochemical analysis of the biomacromolecules and cells. Meanwhile, different electrochemical techniques are employed to meet the requirements of different bioassays.

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Authors and Affiliations

  1. Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, People’s Republic of China

    Genxi Li

  2. Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, People’s Republic of China

    Peng Miao

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  1. Genxi Li
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  2. Peng Miao
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Correspondence to Genxi Li .

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Li, G., Miao, P. (2013). Theoretical Background of Electrochemical Analysis. In: Electrochemical Analysis of Proteins and Cells. SpringerBriefs in Molecular Science. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-34252-3_2

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